Tetrapleura tetraptera curtails oxidative and proinflammatory biochemical events in lithium-pilocarpine model of status epilepticus

IF 1.8 Q3 PHARMACOLOGY & PHARMACY
Comfort O. Oladejo, Omowumi O. Ogundele, Abimbola R. Adeoti, Jesutola R. Atilola, M. Tolulope Olaleye, Afolabi C. Akinmoladun
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引用次数: 0

Abstract

Tetrapleura tetraptera Taub. (Fabaceae), commonly known as Aidan is ethnopharmacologically used for the management of health conditions such as diabetes mellitus, leprosy, epilepsy, and stroke in Nigeria. This study evaluated the anti-oxidoinflammatory properties of T. tetraptera methanol leaf extract (TTE) in lithium-pilocarpine-induced status epilepticus in Wistar rats. The extract was phytochemically screened and HPLC fingerprinting was performed. Animals were intraperitoneally administered with 127 mg/kg lithium chloride followed by 25 mg/kg pilocarpine 20 h later to induce status epilepticus. The animals were post treated with 50, 100 and 200 mg/kg TTE with 10 mg/kg valproic acid as the reference standard drug. Phytochemical screening of TTE confirmed the presence of tannins, phenols, alkaloids, terpenoids, flavonoids, saponins, glycosides and steroids. Quantitative phytochemical and antioxidant analyses of the extract indicated significant in vitro antioxidant and radical scavenging activity. HPLC analysis of the extract confirmed the presence of aridanin and polyphenols. TTE ameliorated redox imbalance by increasing markers for oxidative stress such as ferric reducing antioxidant power, glutathione level, catalase, glutathione S-transferase and superoxide dismutase activities. Moreover, TTE ameliorated pro-inflammatory events by reducing the level of the pro-inflammatory mediator nitric oxide, attenuating lipid peroxidation (which produces inflammatory lipid peroxidation-derived aldehydes), and decreasing the activities of xanthine oxidase and lactate dehydrogenase in the brain. These results indicated that the leaf of Tetrapleura tetraptera has therapeutic potential against status epilepticus by reversing oxidoinflammatory events. Tetrapleura tetraptera leaf extracts could be used to produce novel plant-based pharmaceuticals for treating status epilepticus and associated disorders.

Graphic abstract

Abstract Image

在锂匹罗卡品癫痫持续状态模型中,四翅目四胸膜减少氧化和促炎生化事件
四胸膜动物(Fabaceae),通常被称为Aidan,在民族药理学上用于尼日利亚的糖尿病、麻风病、癫痫和中风等健康状况的管理。本研究评价了四夜蛾甲醇叶提取物(TTE)对锂-匹罗卡品诱导的Wistar大鼠癫痫持续状态的抗氧化炎症作用。对提取物进行植物化学筛选,并进行HPLC指纹图谱分析。动物腹腔注射127 mg/kg氯化锂,20 h后再注射25 mg/kg匹罗卡品诱导癫痫持续状态。分别给予50、100、200 mg/kg的TTE和10 mg/kg的丙戊酸作为标准药物。TTE的植物化学筛选证实存在单宁、酚类、生物碱、萜类、黄酮类、皂苷、糖苷和类固醇。定量的植物化学和抗氧化分析表明,提取物具有显著的体外抗氧化和自由基清除活性。高效液相色谱分析证实该提取物中含有芳旦苷和多酚。TTE通过增加氧化应激标志物如铁还原抗氧化能力、谷胱甘肽水平、过氧化氢酶、谷胱甘肽s -转移酶和超氧化物歧化酶活性来改善氧化还原失衡。此外,TTE通过降低促炎介质一氧化氮的水平,减轻脂质过氧化(产生炎症性脂质过氧化衍生醛),降低脑中黄嘌呤氧化酶和乳酸脱氢酶的活性来改善促炎事件。这些结果表明,四翅四层叶通过逆转氧化炎症事件具有治疗癫痫持续状态的潜力。四翅四胸叶提取物可用于生产治疗癫痫持续状态及相关疾病的新型植物性药物。图形抽象
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来源期刊
Advances in Traditional Medicine
Advances in Traditional Medicine PHARMACOLOGY & PHARMACY-
CiteScore
4.30
自引率
0.00%
发文量
50
期刊介绍: Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
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