The Use of Immunochromatographic Technique for Rotavirus Detection: Experience from a Tertiary Care Hospital in Central Greece

IF 3.2 4区 医学 Q3 VIROLOGY
Intervirology Pub Date : 2019-09-05 DOI:10.1159/000502007
A. Vontas, C. Hadjicristodoulou, Vasilis Krikelis, E. Petinaki
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引用次数: 0

Abstract

Despite the significant medical advances which have taken place in the last decades, acute diarrhoea cases remain a public health issue of major significance, with gastroenteritis agents being associated with severe symptoms in adults and high morbidity in infants and children. Regarding rotaviruses, while children are the predominant victims of rotavirus infection, adults (often caretakers or parents of these children) may experience the same symptoms of fever, vomiting, and non-bloody diarrhoea. Three different routine schemes for the detection of rotaviruses in archived stool samples were evaluated in terms of diagnostic performance. A total of 640 archived stool samples were included in the study. The samples were screened with three different techniques: a commercial rapid immunochromatographic test, a modified in-house conventional one-step reverse-transcription polymerase chain reaction (PCR) screen protocol, and a commercial one-step real-time PCR kit. Technical aspects and considerations are discussed.
免疫色谱技术在轮状病毒检测中的应用:希腊中部一家三级医院的经验
尽管在过去几十年中取得了重大的医学进步,但急性腹泻病例仍然是一个重要的公共卫生问题,肠胃炎药物与成人的严重症状和婴儿和儿童的高发病率有关。关于轮状病毒,虽然儿童是轮状病毒感染的主要受害者,但成年人(通常是这些儿童的看护人或父母)可能会出现同样的发烧、呕吐和无血腹泻症状。根据诊断性能评估了三种不同的常规方案,用于检测存档粪便样本中的轮状病毒。研究共包括640份存档的粪便样本。用三种不同的技术对样本进行筛选:商业快速免疫层析检测、改良的内部常规一步逆转录聚合酶链式反应(PCR)筛选方案和商业一步实时PCR试剂盒。讨论了技术方面的问题和注意事项。
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来源期刊
Intervirology
Intervirology 医学-病毒学
CiteScore
5.40
自引率
0.00%
发文量
13
审稿时长
6-12 weeks
期刊介绍: ''Intervirology'' covers progress in both basic and clinical virus research, and aims to provide a forum for the various disciplines within virology. Issues publishing original papers alternate with thematic issues, focusing on clearly defined topics. This thematic concentration serves to make timely reviews, research reports and controversy easily accessible to both specialists in the field and those who want to keep track of the latest developments outside their own area of interest. In addition to original papers, regular issues publish short communications and letters to the editor to provide readers with a forum for the exchange of ideas and comments. The scope encompasses work on the molecular biology of human and animal viruses, including genome organization and regulation, and the structure and function of viral proteins. The pathogenesis, immunology, diagnosis, epidemiology, prophylaxis and therapy of viral diseases are considered.
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