Lacticaseibacilli and Lactococci from Slovak Raw Goat Milk and their Potential

Abstract

Abstract Strains potential such as bacteriocin activity, biofilm formation ability, growth in skim milk, susceptibility to antibiotics, tolerance to bile and low pH as well as enzyme production was tested in the species Lacticaseibacillus paracasei and Lactococcus lactis detected in Slovak raw goat milk. The strains showed mostly low-grade biofilm formation ability, susceptibility to antibiotics and sufficient tolerance to oxgall/bile. Lacticaseibacillus (Lcb.) paracasei ZM-1, ZM-2 and Lactococcus (Lc.) lactis PD MO 1/8 showed high tolerance to pH 3 (67 %, 83 % and 63 %, respectively). The strains showed bacteriocin activity against the principal indicator Enterococcus avium EA5 (inhibition zone ranging 5–24 mm). A concentrated substance of Lcb. paracasei LPa ML 12/1 (pH 6.3) inhibited EA5 strain (inhibition activity 100 AU ml–1). Lcb. paracasei ZM-1 and ZM-2 also produced a high amount of β-galactosidase (40 nmol). Although the strains indicated their beneficial potential, additional testing is needed; some tests are in processing for further possible application of selected strains in dairy.