An in vitro platform for study of the human gut microbiome under an oxygen gradient

IF 3 4区医学 Q3 ENGINEERING, BIOMEDICAL

Biomedical Microdevices Pub Date : 2023-04-04 DOI:10.1007/s10544-023-00653-3

James Comolli, David I. Walsh III, Johanna Bobrow, Chelsea L. Lennartz, Nicholas J. Guido, Todd Thorsen

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引用次数: 1

Abstract

The complex, dynamic environment of the human lower gastrointestinal tract is colonized by hundreds of bacterial species that impact health and performance. Ex vivo study of the functional interactions between microbial community members in conditions representative of those in the gut is an ongoing challenge. We have developed an in vitro 40-plex platform that provides an oxygen gradient to support simultaneous maintenance of microaerobic and anaerobic microbes from the gut microbiome that can aid in rapid characterization of microbial interactions and direct comparison of individual microbiome samples. In this report, we demonstrate that the platform more closely maintained the microbial diversity and composition of human donor fecal microbiome samples than strict anaerobic conditions. The oxygen gradient established in the platform allowed the stratification and subsequent sampling of diverse microbial subpopulations that colonize microaerobic and anaerobic micro-environments. With the ability to run forty samples in parallel, the platform has the potential to be used as a rapid screening tool to understand how the gut microbiome responds to environmental perturbations such as toxic compound exposure, dietary changes, or pharmaceutical treatments.

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在氧梯度下研究人类肠道微生物组的体外平台

人类下胃肠道复杂的动态环境是由数百种影响健康和性能的细菌定植的。在具有代表性的肠道条件下，微生物群落成员之间功能相互作用的离体研究是一项持续的挑战。我们开发了一种体外40-plex平台，该平台提供氧气梯度，支持同时维持肠道微生物组中的微氧和厌氧微生物，有助于快速表征微生物相互作用和直接比较单个微生物组样品。在本报告中，我们证明该平台比严格的厌氧条件更能维持人类供体粪便微生物组样本的微生物多样性和组成。在平台上建立的氧梯度允许在微氧和厌氧微环境中定植的不同微生物亚群的分层和后续采样。由于能够同时运行40个样本，该平台有可能被用作快速筛选工具，以了解肠道微生物组对环境扰动(如有毒化合物暴露、饮食变化或药物治疗)的反应。

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来源期刊

Biomedical Microdevices 工程技术-工程：生物医学

CiteScore

6.90

自引率

3.60%

发文量

审稿时长

6 months

期刊介绍： Biomedical Microdevices: BioMEMS and Biomedical Nanotechnology is an interdisciplinary periodical devoted to all aspects of research in the medical diagnostic and therapeutic applications of Micro-Electro-Mechanical Systems (BioMEMS) and nanotechnology for medicine and biology. General subjects of interest include the design, characterization, testing, modeling and clinical validation of microfabricated systems, and their integration on-chip and in larger functional units. The specific interests of the Journal include systems for neural stimulation and recording, bioseparation technologies such as nanofilters and electrophoretic equipment, miniaturized analytic and DNA identification systems, biosensors, and micro/nanotechnologies for cell and tissue research, tissue engineering, cell transplantation, and the controlled release of drugs and biological molecules. Contributions reporting on fundamental and applied investigations of the material science, biochemistry, and physics of biomedical microdevices and nanotechnology are encouraged. A non-exhaustive list of fields of interest includes: nanoparticle synthesis, characterization, and validation of therapeutic or imaging efficacy in animal models; biocompatibility; biochemical modification of microfabricated devices, with reference to non-specific protein adsorption, and the active immobilization and patterning of proteins on micro/nanofabricated surfaces; the dynamics of fluids in micro-and-nano-fabricated channels; the electromechanical and structural response of micro/nanofabricated systems; the interactions of microdevices with cells and tissues, including biocompatibility and biodegradation studies; variations in the characteristics of the systems as a function of the micro/nanofabrication parameters.