Bioactive Fraction of Mentha piperita Leaves Extract Stimulates Lipoprotein Lipase at Cellular and Molecular Levels via Augmentation of Antioxidant Defense System in Hyperlipidemic Rats

IF 1.2 Q4 PHARMACOLOGY & PHARMACY

Journal of Herbs, Spices and Medicinal Plants Pub Date : 2021-03-01 DOI:10.1080/10496475.2021.1891176

H. Mohammed, A. Sallau, Humphrey Chukwu Nzelibe, A. Muhammad, O. Erukainure, B. Musa, M. K. Anigo

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引用次数: 1

Abstract

ABSTRACT The antihyperlipidemic and antioxidant effects of Mentha piperita leaves extracts in Poloxamer 407-induced hyperlipidemic rats were investigated. Antihyperlipidemic activities of hexane, ethyl acetate, and methanol extracts (100, 200, or 300 mg kg−1 b.w.) were evaluated by lipid profile analysis. Twelve (12) fractions (F1 to F12) were collected from the most antihyperlipidemic extract (ethyl acetate at 100 mg kg−1) and, assayed in vivo for antihyperlipidemic activity. The effects of the most antihyperlipidemic fraction (F2) on redox sensitive biomarkers (SOD, CAT, GSH, MDA) and lipoprotein lipase (LPL) activity at cellular and molecular levels were evaluated. Fraction-2 (F2) was further subjected to GC-MS and LC-MS analysis for identification of nonpolar and polar components. The F2 treatment decreased the level of MDA with concomitant enhancement of GSH, LPL activity in the liver and serum and up regulated the expression of SOD, CAT, and LPL genes in liver and blood relative to the untreated rats. GC-MS and LC-MS revealed oleic acid, vaccenic acid, 3, 4-dicaffeoylquinic acid, coumarin, and triacontane as the probable bioactive components with reported antihyperlipidemic and antioxidant effects.

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薄荷叶提取物生物活性组分通过增强高脂血症大鼠抗氧化防御系统在细胞和分子水平上刺激脂蛋白脂肪酶

摘要:研究薄荷叶提取物对poloxam407诱导的高脂血症大鼠的降血脂和抗氧化作用。通过脂质谱分析评估己烷、乙酸乙酯和甲醇提取物(100、200或300 mg kg - 1 b.w)的降血脂活性。从最有效的抗高血脂提取物(乙酸乙酯浓度为100 mg kg - 1)中收集12 (F1至F12)个部分，并在体内测定其抗高血脂活性。在细胞和分子水平上评估了抗高脂血症部分(F2)对氧化还原敏感生物标志物(SOD、CAT、GSH、MDA)和脂蛋白脂肪酶(LPL)活性的影响。部分-2 (F2)进一步进行GC-MS和LC-MS分析，以鉴定非极性和极性成分。与未处理大鼠相比，F2处理降低了MDA水平，同时提高了肝脏和血清中GSH、LPL活性，上调了肝脏和血液中SOD、CAT和LPL基因的表达。GC-MS和LC-MS显示油酸、异丙酸、3,4 -二咖啡酰奎宁酸、香豆素和三康烷可能是具有抗高脂血症和抗氧化作用的生物活性成分。

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.