Staining procedure to identify paddy with gaps vulnerable to Stenotus rubrovittatus and Trigonotylus caelestialium (Hemiptera: Miridae)

IF 1.3 4区 农林科学 Q2 ENTOMOLOGY
Hiroaki Takeuchi
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Abstract

Stenotus rubrovittatus (Matsumura) and Trigonotylus caelestialium (Kirkaldy) (Hemiptera: Miridae) are major rice pests in Japan. Since both species feed on rice grain through the gap between the lemma and palea, the paddy grains with gap (including split-hull) is vulnerable to them. The aim of this study was to develop a staining procedure to identify the vulnerable paddy with gap. The paddy samples were first stained under different conditions to select the optimal staining conditions that could highlight the gap and help sort the split-hull paddy clearly by visual inspection. After optimization experiments, 0.05% aqueous methylene blue solution was selected for staining, and 3 min was set as the staining and pretreatment times. The efficiency of this staining procedure was evaluated by applying it to the paddy harvested from fields to examine how well it could sort the vulnerable paddy. The stained paddy percentages were similar in the five replicates for the staining test, thereby confirming its reliability. The gap detection rate determined by the staining technique was approximately 30% higher than that determined by visual inspection. These results indicate that the staining technique enables the identification of vulnerable paddy, and may be useful for estimating vulnerable product and analyzing potential damage.

Abstract Image

用染色法鉴定易受红纹窄尾虫和盲肠三趾虫侵害的间隙水稻(半翅目:Miridae)
日本水稻害虫中主要有两种,一种是松村螟(stotus rubrovitatus),另一种是柯卡迪螟(triconotylus caelestialium Kirkaldy)。由于这两种水稻都是通过外稃与旧叶之间的间隙取食稻谷的,因此有间隙(包括裂壳)的稻谷对它们很脆弱。本研究的目的是建立一种染色方法来鉴定有间隙的脆弱水稻。首先在不同的染色条件下对稻壳样品进行染色,选择最优的染色条件,使裂壳稻壳的缝隙突出,便于目测清晰分选。优化实验后,选择0.05%亚甲蓝水溶液进行染色,3 min为染色和预处理时间。通过将该染色方法应用于田间收获的水稻来评估其对易损水稻的分类效果。染色试验中5个重复的水稻染色百分率相似,证实了其可靠性。通过染色技术确定的间隙检出率比目测确定的间隙检出率高约30%。这些结果表明,该染色技术能够有效地鉴定水稻易损品种,并可用于易损产品的鉴定和潜在危害分析。
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来源期刊
CiteScore
2.70
自引率
7.70%
发文量
37
审稿时长
6-12 weeks
期刊介绍: Applied Entomology and Zoology publishes articles concerned with applied entomology, applied zoology, agricultural chemicals and pest control in English. Contributions of a basic and fundamental nature may be accepted at the discretion of the Editor. Manuscripts of original research papers, technical notes and reviews are accepted for consideration. No manuscript that has been published elsewhere will be accepted for publication.
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