Cowpea transcriptional reprogramming during two different physiological moments of root dehydration

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2022-12-01 DOI:10.1016/j.plgene.2022.100374

José Ribamar Costa Ferreira-Neto , Artemisa Nazaré Costa Borges , Manassés Daniel da Silva , David Anderson de Lima Morais , Valesca Pandolfi , Antônio Félix da Costa , Fabiana Aparecida Rodigues , Alexandre Lima Nepomuceno , Ana Maria Benko-Iseppon

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引用次数: 0

Abstract

The transcriptomes of two distinct physiological moments of root dehydration condition were scrutinized in cowpea. The RD25 (first 25 min after root dehydration imposition) physiological data did not indicate significant alterations. For the other treatment, 150 min under root dehydration (RD150), all physiological data indicated that the studied cultivar was under stress. The physiological differences between RD25 and RD150 reverberated in the respective transcriptomes. The sets of in silico differentially expressed isoforms showed specificity for each treatment time. The comparison of T25 | UR [up-regulated transcripts in T25 (RD25 vs. Cont25)] vs. T150 | UR [up-regulated transcripts in T150 (RD150 vs. Cont150)] enriched GO terms (associated with abiotic stresses), despite certain similarities, showed us that they were associated with the respective physiological moments. Concerning gene families, a large portion of those present in the T25 | UR were associated with signaling processes; for T150 | UR, a miscellany of families (from transcription factors to nonenzymatic proteins) was observed. The plotting of transcriptomics data in the KEGG Pathway database indicated a change in the topology of activated metabolic modules in T25 | UR vs. T150 | UR. For the latter, it was observed that most activated modules were associated with specialized metabolism. C2H2 and BPC1 transcription factors (TFs) sites were enriched at T25 | UR and T150 | UR gene promoters, suggesting the importance of these TFs for cowpea response to root dehydration. Our work provides insights into specific molecular actors and pathways, enhancing our global understanding of cowpea stress response.

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豇豆根脱水两个不同生理时刻的转录重编程

对豇豆根系脱水两个不同生理时刻的转录组进行了研究。RD25(根脱水后的前25分钟)生理数据没有明显变化。在另一个处理150min (RD150)下，所有生理数据都表明所研究品种处于胁迫状态。RD25和RD150之间的生理差异反映在各自的转录组中。不同处理时间的硅片差异表达异构体具有特异性。T25 | UR [T25中的上调转录本(RD25 vs. Cont25)]与T150 | UR [T150中的上调转录本(RD150 vs. Cont150)]富集氧化氧化烯(与非生物胁迫相关)的比较，尽管有一定的相似性，但表明它们与各自的生理时刻相关。在基因家族方面，T25 | UR中存在的大部分基因与信号传导过程有关;对于T150 | UR，观察到各种家族(从转录因子到非酶蛋白)。KEGG Pathway数据库中的转录组学数据显示，T25 | UR与T150 | UR中活化代谢模块的拓扑结构发生了变化。对于后者，我们观察到大多数激活的模块与专门的代谢有关。C2H2和BPC1转录因子(TFs)位点富集在T25 | UR和T150 | UR基因启动子上，表明这些转录因子在豇豆对根系脱水的响应中具有重要作用。我们的工作提供了对特定分子因子和途径的见解，增强了我们对豇豆应激反应的全球理解。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.