Isolation of phytoconstituents and evaluation of biological potentials of Berberis hispanica from Algeria

IF 0.9 4区医学 Q4 PHARMACOLOGY & PHARMACY

Bangladesh Journal of Pharmacology Pub Date : 2018-06-07 DOI:10.3329/BJP.V13I2.36133

R. Lemoui, S. Benyahia, Labib Noman, Ilham Bencherchar, F. Oke-Altuntas, K. Rebbas, S. Benayache, F. Benayache, I. Demirtaş

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引用次数: 7

Abstract

The aim of this study was to isolate the phytoconstituents and evaluate the biological activities of Berberis hispanica. Three phenolic compounds (tamarixetin, caffeic acid and rutin) were isolated from B. hispanica. The structures of the pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D-, 2D-NMR, and HPLC-TOF/MS. In addition, antimicrobial, anti-oxidant and anti-proliferative effects of the extracts, some fractions and isolated compounds were evaluated. The extracts of B. hispanica were evaluated against six bacterial strains and exhibited the highest activity against Klebsiella pneumonia (15 mm at 100 mg/mL). Fraction T36 (IC₅₀<5 μg/mL) from the n-butanol extract displayed higher radical scavenging activity than butylated hydroxytoluene. The isolated compounds were evaluated for their antiproliferative effects against human cervical adenocarcinoma (HeLa) cell line by real time cell analyzer assay and tamarixetin exhibited the remarkable effect (IC₅₀<50 μg/mL) on HeLa cells. This study supports the documented medicinal effects of B. hispanica.

Video Clip of Methodology:

Antiproliferative Assay: 4 min 31 sec Full Screen Alternate

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阿尔及利亚小檗的植物成分分离及生物学潜力评价

本研究的目的是分离西班牙小檗的植物成分并评价其生物活性。从西班牙白杨中分离到3个酚类化合物(塔玛西汀、咖啡酸和芦丁)。通过1D- nmr、2D-NMR、HPLC-TOF/MS等光谱和质谱分析对化合物的结构进行了鉴定。此外，还对其提取物、部分组分及分离化合物的抗菌、抗氧化、抗增殖作用进行了评价。结果表明，在100 mg/mL浓度下，对肺炎克雷伯氏菌的抑菌活性最高(15 mm)。分数T36 (IC5050B)hispanica。方法视频剪辑:抗增殖试验:4分31秒全屏交替播放

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来源期刊

Bangladesh Journal of Pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

1.50

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS). A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements. Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review. Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.