Genetic Diversity of Duabanga moluccana Blume from Two Provenances in West Nusa Tenggara Revealed by Microsatellite markers

S. Larekeng, Yusniar Yusniar, M. Restu, R. Rismawati, Y. F. Cahyaningsih, M. A. Arsyad, A. Nirsatmanto
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引用次数: 2

Abstract

Duabanga moluccana Blume, locally known as Rajumas, is a tree species that suitable for building materials, pulp, and plywood. The information about genetic diversity is required for the conserving of this species. Here, we elucidated the genetic diversity of D. moluccana from two provenances in West Nusa Tenggara, Indonesia. Four microsatellite markers successfully amplified 12 randomly selected samples and produced polymorphic DNA bands. Those primers were DMAG10, DMAG09, DMACAG01 and DMTCAC11. High genetic diversity was detected in the populations with 0.54 of mean expected heterozygosity (He). The genetic variation among individuals was 100%, whereas there were no genetic variations among populations and within individuals
利用微卫星标记揭示了西努沙登加拉两个种源的杜阿班加·摩鹿加纳(Duabanga moluccana Blume)遗传多样性
Duabanga moluccana Blume,在当地被称为Rajumas,是一种适合建筑材料,纸浆和胶合板的树种。遗传多样性的信息是保护该物种所必需的。本文对印度尼西亚西努沙登加拉两个种源的moluccana的遗传多样性进行了分析。4个微卫星标记成功扩增了12个随机选择的样本,并产生了多态性DNA条带。引物分别为DMAG10、DMAG09、DMACAG01和DMTCAC11。群体遗传多样性较高,平均期望杂合度(He)为0.54。个体间遗传变异为100%,群体间和个体内无遗传变异
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