Effect of Diclofenac and Andrographolide Combination on Carrageenan-Induced Paw Edema and Hyperalgesia in Rats

IF 2.3 4区医学 Q3 PHARMACOLOGY & PHARMACY

Dose-Response Pub Date : 2022-04-01 DOI:10.1177/15593258221103846

Augustine Tandoh, C. Danquah, C. Benneh, D. W. Adongo, Eric Boakye-Gyasi, E. Woode

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引用次数: 4

Abstract

Studies into drug combination at low doses are a promising approach to the management of pain and inflammation. The aim of this study was to evaluate the anti-edema and anti-hyperalgesic effects of a combination of diclofenac and andrographolide. Male Sprague-Dawley rats were first treated with diclofenac or andrographolide alone (3–100 mg/kg), as well as a combination of the 2 drugs. Carrageenan was then injected into the right hind paw of rats, and changes in paw volume and sensitivity to mechanical (von Frey) and thermal (Hargreaves test) stimuli measured. Results showed drug combination produced synergistic effects at reducing paw edema especially at lower doses, with a Loewe synergy score of 13.02 ± 8.75 in SynergyFinder and a combination index of .41 ± .18 after isobolographic analysis. Again synergy scores for decreasing response to 1.0 and 3.6 g force application of von Frey filaments after drug combination were 10.127 ± 5.68 and 8.554 ± 6.53, respectively, in SynergyFinder. Synergistic effects were also seen after drug combination in the Hargreaves test with a synergy score of 5.136 ± 16.38. In conclusion, combination of diclofenac with andrographolide showed better pharmacologic effects after carrageenan injection and was more synergistic at low-dose combinations.

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双氯芬酸和穿心莲内酯联合用药对卡拉胶致大鼠爪水肿和痛觉过敏的影响

对低剂量药物组合的研究是治疗疼痛和炎症的一种很有前途的方法。本研究的目的是评估双氯芬酸和穿心莲内酯联合用药的抗水肿和抗痛觉过敏作用。雄性Sprague-Dawley大鼠首先用双氯芬酸或穿心莲内酯单独（3-100 mg/kg）以及这两种药物的组合进行治疗。然后将卡拉胶注射到大鼠的右后爪中，并测量爪体积和对机械（von Frey）和热（Hargreaves试验）刺激的敏感性的变化。结果显示，药物组合在减少爪水肿方面产生协同作用，尤其是在低剂量时，SynergyFinder中的Loewe协同得分为13.02±8.75，等辐射热分析后的组合指数为.41±.18。在SynergyFinder中，药物组合后对1.0 g和3.6 g von Frey细丝施加力的反应降低的协同作用得分分别为10.127±5.68和8.554±6.53。在哈格里夫斯试验中，药物组合后也出现了协同作用，协同作用得分为5.136±16.38。总之，卡拉胶注射后，双氯芬酸与穿心莲内酯的组合显示出更好的药理作用，并且在低剂量组合中具有更强的协同作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dose-Response PHARMACOLOGY & PHARMACY-RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

CiteScore

4.90

自引率

4.00%

发文量

140

审稿时长

>12 weeks

期刊介绍： Dose-Response is an open access peer-reviewed online journal publishing original findings and commentaries on the occurrence of dose-response relationships across a broad range of disciplines. Particular interest focuses on experimental evidence providing mechanistic understanding of nonlinear dose-response relationships.