ARF GTPase machinery at the plasma membrane regulates auxin transport-mediated plant growth.

IF 1.4 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Plant Biotechnology Pub Date : 2018-06-25 DOI:10.5511/PLANTBIOTECHNOLOGY.18.0312A

S. Naramoto, J. Kyozuka

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引用次数: 11

Abstract

VAN3 is a plant ACAP-type ADP-ribosylation factor-GTPase activating protein (ARF-GAP) that regulates auxin transport-mediated plant morphogenesis such as continuous venation and lateral root development in Arabidopsis. Previous studies suggested that VAN3 localizes at the plasma membrane (PM) and intracellular structures. However, the role of PM localization in mediating the van3 mutant phenotype is not clear. Here we performed subcellular localization analysis of VAN3 and its regulators CVP2 and VAB to determine their endogenous functions. We found that GFP-tagged CVP2 and VAB preferentially localize at the PM in stably transformed plants. We determined that transgenic plants with lower expression levels of GFP- or mRFP-tagged VAN3 displayed PM localization, which was sufficient to rescue the van3 mutant. Functional VAN3-mRFP and VAB-GFP colocalized at PMs. The van3 mutant phenotype was suppressed by mutation of VAN7/GNOM, which encodes an ARF-GEF that localizes at the PM and Golgi apparatus. These combined results suggest that ARF-GTPase machinery at the PM regulates auxin transport-mediated plant growth and development.

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质膜上的ARF GTP酶机制调节生长素转运介导的植物生长。

VAN3是一种植物acap型adp -核糖基化因子- gtpase激活蛋白(ARF-GAP)，在拟南芥中调节生长素运输介导的植物形态发生，如连续脉化和侧根发育。先前的研究表明，VAN3定位于质膜(PM)和细胞内结构。然而，PM定位在介导van3突变表型中的作用尚不清楚。本研究对VAN3及其调控因子CVP2和VAB进行了亚细胞定位分析，以确定其内源性功能。我们发现gfp标记的CVP2和VAB在稳定转化的植物中优先定位于PM。我们确定GFP-或mrfp -标记的VAN3表达水平较低的转基因植物显示PM定位，这足以拯救VAN3突变体。功能性VAN3-mRFP和VAB-GFP共定位于pm。van3突变表型被VAN7/GNOM突变抑制，该突变编码位于PM和高尔基体的ARF-GEF。这些综合结果表明，PM上的ARF-GTPase机制调节生长素运输介导的植物生长和发育。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-PLANT SCIENCES

CiteScore

2.90

自引率

18.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Plant Biotechnology is an international, open-access, and online journal, published every three months by the Japanese Society for Plant Biotechnology. The journal, first published in 1984 as the predecessor journal, “Plant Tissue Culture Letters” and became its present form in 1997 when the society name was renamed to Japanese Society for Plant Cell and Molecular Biology, publishes findings in the areas from basic- to application research of plant biotechnology. The aim of Plant Biotechnology is to publish original and high-impact papers, in the most rapid turnaround time for reviewing, on the plant biotechnology including tissue culture, production of specialized metabolites, transgenic technology, and genome editing technology, and also on the related research fields including molecular biology, cell biology, genetics, plant breeding, plant physiology and biochemistry, metabolic engineering, synthetic biology, and bioinformatics.