In vitro repress of breast cancer by bio-product of edible Pleurotus ostreatus loaded with chitosan nanoparticles

IF 2.3 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Aisha M. H. Al-Rajhi, Tarek M. Abdel Ghany
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引用次数: 2

Abstract

Despite advances in early detection and therapy, cancer still is a significant health challenge with the highest priority for investigation. Breast cancer represents the most common cancerous disease among women in the world. The study’s purpose is to estimate the cytotoxic activity of the edible mushroom Pleurotus ostreatus extract (PE), chitosan nanoparticles (ChNPs), and PE loaded with ChNPs (PELChNPs), as well as to identify the molecular docking of the cytotoxicity of methyl gallate (MG) as a main component of the PE against breast cancer (MCF-7) cell line. High-performance liquid chromatography (HPLC) analysis of PE exhibited the existence of various phenolic and flavonoid compounds such as MG, gallic acid, chlorogenic acid, hesperetin, naringenin, rutin, and cinnamic acid. The proliferation of the MCF-7 cell line was inhibited at 1, 3.9, and 62.50 µg/mL of PELChNPs, PE, and ChNPs, respectively. PELChNPs were more effective against the MCF-7 cell line than PE, particularly at low concentrations. For instance, at 7.8 µg/mL of PELChNPs and PE, the inhibitory % of MCF-7 proliferation was 20.59±1.75% and 8.57±0.59%, respectively. At 15.6 µg/mL of PELChNPs and PE, the inhibitory % of MCF-7 proliferation was 51.37±1.09% and 25.18±1.64%, respectively. While there is slight difference in the inhibition % of MCF-7 cells (98.64±0.21 and 97.22±0.16%) at high concentration 500 µg/mL of PELChNPs and PE, respectively. IC50 was 15.25 ± 0.54 µg/mL, 46.27 ± 1.94 µg/mL, and 337.38 ± 13.68 µg/mL against MCF-7 cell line of PELChNPs, PE, and ChNPs, respectively. The value of IC50 documented the efficacy of PELChNPs compared with the IC50 (5.91 ± 0.43 µg/mL) of Vinblastine sulfate. Noticeable distortions were observed in the MCF-7 cell line mainly treated with PELChNPs, followed by PE alone. While ChNPs exhibited less effect on the morphology of the MCF-7 cell line. Antioxidant activity of ChNPs, PE, and PELChNPs was evaluated compared with Trolox, which reflected IC50 = 118.33 ± 4.02, 85.63 ± 3.96, 36.80 ± 2.52 and 24.74 ± 0.45 µg/mL. Methyl gallate binding interactions were assessed using molecular docking with the MOE-Dock tool against the target crystal structures of Breast cancer cell line 3HB5. The results shed light on how molecular modeling techniques can inhibit methyl gallate with possible uses in treating breast cancer.

壳聚糖纳米颗粒可食性平菇生物制品对乳腺癌的体外抑制作用
尽管在早期发现和治疗方面取得了进展,但癌症仍然是一个重大的健康挑战,需要进行最优先的研究。乳腺癌是世界上妇女中最常见的癌症。本研究的目的是评估食用菌平菇提取物(Pleurotus ostreatus extract, PE)、壳聚糖纳米颗粒(ChNPs)和负载ChNPs的PE (PELChNPs)的细胞毒活性,并确定作为PE主要成分的没食子酸甲酯(methyl gallate, MG)对乳腺癌细胞系(MCF-7)细胞毒的分子对接。高效液相色谱(HPLC)分析表明,PE中含有MG、没食子酸、绿原酸、橙皮苷、柚皮苷、芦丁和肉桂酸等多种酚类和类黄酮化合物。PELChNPs、PE和ChNPs浓度分别为1、3.9和62.50µg/mL时,MCF-7细胞系的增殖受到抑制。PELChNPs对MCF-7细胞系比PE更有效,特别是在低浓度时。例如,当PELChNPs和PE浓度为7.8µg/mL时,MCF-7的增殖抑制率分别为20.59±1.75%和8.57±0.59%。在15.6µg/mL PELChNPs和PE浓度下,MCF-7的增殖抑制率分别为51.37±1.09%和25.18±1.64%。而在高浓度500µg/mL的PELChNPs和PE对MCF-7细胞的抑制率分别为98.64±0.21和97.22±0.16%,差异有统计学意义。对PELChNPs、PE和ChNPs的MCF-7细胞株的IC50分别为15.25±0.54µg/mL、46.27±1.94µg/mL和337.38±13.68µg/mL。与硫酸长春碱的IC50(5.91±0.43µg/mL)相比,PELChNPs的IC50值记录了其疗效。在主要使用PELChNPs处理的MCF-7细胞系中观察到明显的扭曲,其次是单独使用PE。而ChNPs对MCF-7细胞系形态的影响较小。与Trolox比较,ChNPs、PE和PELChNPs的抗氧化活性分别为118.33±4.02、85.63±3.96、36.80±2.52和24.74±0.45µg/mL。利用MOE-Dock工具对乳腺癌细胞系3HB5的靶晶体结构进行分子对接,评估没食子酸甲酯的结合相互作用。这些结果揭示了分子模拟技术如何抑制没食子酸甲酯在治疗乳腺癌中的可能用途。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Applied Biological Chemistry
Applied Biological Chemistry Chemistry-Organic Chemistry
CiteScore
5.40
自引率
6.20%
发文量
70
审稿时长
20 weeks
期刊介绍: Applied Biological Chemistry aims to promote the interchange and dissemination of scientific data among researchers in the field of agricultural and biological chemistry. The journal covers biochemistry and molecular biology, medical and biomaterial science, food science, and environmental science as applied to multidisciplinary agriculture.
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