Evaluation of Four Malaria Rapid Diagnostic Test Kits Used at the Enyiresi Government Hospital in the Eastern Region of Ghana

IF 1.4 Q3 PARASITOLOGY

Journal of Parasitology Research Pub Date : 2023-03-09 DOI:10.1155/2023/4226020

S. Domfeh, Boateng Y. Darkwa, Raymond K. Gablah, Evans Adu-Asamoah, C. Obirikorang

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引用次数: 0

Abstract

Microscopic identification of Plasmodium spp. is the gold standard for malaria diagnosis. However, malaria rapid diagnostic test kits are also available for prompt diagnosis. This study evaluated four routinely used malaria rapid diagnostic test kits at the Enyiresi Government Hospital in the Eastern Region of Ghana. This cross-sectional study was conducted among 238 patients suspected of malaria. Venous blood samples were collected to identify Plasmodium falciparum using microscopic techniques. Further, the performances of four malaria rapid diagnostic test kits, First Response Malaria Ag Pf, Carestart Malaria Pf, SD Bioline Ag Pf, and ABON Malaria Pf, were evaluated using the results from the microscopy as the standard reference. As confirmed by microscopy, 65.5% (156/238) of the patients have falciparum malaria. All malaria rapid diagnostic test kits had sensitivities and specificities over 75% compared to microscopy results as the reference standard. However, the SD Bioline Ag Pf kit recorded the highest agreement with the microscopy ( Cohe n ’ s kappa = 0.879 ). All the malaria rapid diagnostic test kits performed quite well and can be used in emergencies. However, results from these kits need to be confirmed by microscopy.

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评价加纳东部地区恩尼雷西政府医院使用的四种疟疾快速诊断检测试剂盒

疟原虫的显微镜鉴定是诊断疟疾的金标准。然而，疟疾快速诊断试剂盒也可用于及时诊断。这项研究评估了加纳东部地区Enyiresi政府医院四个常规使用的疟疾快速诊断试剂盒。这项横断面研究是在238名疑似疟疾患者中进行的。采集静脉血样，用显微镜技术鉴定恶性疟原虫。此外，使用显微镜的结果作为标准参考，评估了四种疟疾快速诊断试剂盒的性能，即第一反应疟疾Ag-Pf、Carestart疟疾Pf、SD Bioline Ag-Pf和ABON疟疾Pf。显微镜检查证实，65.5%（156/238）的患者患有恶性疟疾。与作为参考标准的显微镜结果相比，所有疟疾快速诊断试剂盒的敏感性和特异性均超过75%。然而，SD Bioline Ag-Pf试剂盒与显微镜的一致性最高（Cohen n’s κ=0.879）。所有的疟疾快速诊断试剂盒都表现良好，可以在紧急情况下使用。然而，这些试剂盒的结果需要通过显微镜进行确认。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Parasitology Research Medicine-Infectious Diseases

CiteScore

3.50

自引率

9.10%

发文量

审稿时长

13 weeks

期刊介绍： Journal of Parasitology Research is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of basic and applied parasitology. Articles covering host-parasite relationships and parasitic diseases will be considered, as well as studies on disease vectors. Articles highlighting social and economic issues around the impact of parasites are also encouraged. As an international, Open Access publication, Journal of Parasitology Research aims to foster learning and collaboration between countries and communities.