Systemic Redox Status of Rats Treated with Different Doses of Perfluorocarbon Based Blood Substitute- Perftoran®

Abstract

Abstract The aim of this research was to examine the influence of the intraperitoneal application of PFT in different doses and regimen on systemic oxidative stress and activity of antioxidative enzymes in animals. Depending on whether the animals received only saline or PFT in different doses (8, 12, 16 ml/kg body weight), and time (1, 10, or 20 hours before sacrificing and blood sampling), all animals were divided into control or experimental groups. From plasma samples we measured following biomarkers of oxidative stress: superoxide anion radical (O2−), hydrogen peroxide (H2O2), nitrites (NO2−), index of lipid peroxidation measured as TBARS (thiobarbituric acid reactive substances), and from hemolysate samples activity of the next enzymes: catalase (CAT), superoxidedismutase (SOD) and reduced glutathione (GSH). All mentioned biochemical parameters of oxidative stress were determined spectrophotometrically (Shimadzu UV-1800UV-VIS spectrophotometer, Japan). Superoxide anion radical was a molecule very affected with the PFT administration. we observed the significantly higher activity of superoxide dismutase in all PFT treated groups in comparison with the CTRL group. The highest activity was observed in group treated with the 8 and 12 ml/kg of PFT nearly to sampling (1 hour). Catalase activity was significantly higher in PFT group in comparison with the CTRL, especially in PFT 16ml/kg group (1 hour). In comparison with the CTRL group, the total content of GSH was significantly lower in the groups treated PFT in dose of 16 ml/kg 1 hour and 10 hours before blood sampling. All these changes in oxidative stress markers seems to be very clear, but we can observe that almost all changes are induced in 1 hour after PFT administration. Probably, PFT solution has short-term protective effects on reducing oxidative stress, but no long term-effects. Maybe the chemical and biological instability of PFT solution could be a reason for that transient antioxidative effects, and developing the nano-formulation of PFT could be potential option for resolving the problem with poor pharmacodynamic of PFT.