A new class of teraryl-based AIEgen for highly selective imaging of intracellular lipid droplets and its detection in advanced-stage human cervical cancer tissues†

IF 6.1 3区医学 Q1 MATERIALS SCIENCE, BIOMATERIALS

Journal of Materials Chemistry B Pub Date : 2023-09-29 DOI:10.1039/D3TB01764H

Chandra Prakash Sharma, Akanksha Vyas, Priyanka Pandey, Shashwat Gupta, Ravi Prakash Vats, Sakshi Priya Jaiswal, Madan Lal Brahma Bhatt, Monika Sachdeva and Atul Goel

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Abstract

Lipid droplets (LDs) have drawn much attention in recent years. They serve as the energy reservoir of cells and also play an important role in numerous physiological processes. Furthermore, LDs are found to be associated with several pathological conditions, including cancer and diabetes mellitus. Herein, we report a new class of teraryl-based donor–acceptor-appended aggregation-induced emission luminogen (AIEgen), 6a, for selective staining of intracellular LDs in in vitro live 3T3-L1 preadipocytes and the HeLa cancer cell line. In addition, AIEgen 6a was found to be capable of staining and quantifying the LD accumulation in the tissue sections of advanced-stage human cervical cancer patients. Unlike commercial LD staining dyes Nile Red, BODIPY and LipidTOX, AIEgen 6a showed a high Stokes shift (195 nm), a good fluorescence lifetime decay of 12.7 ns, and LD staining persisting for nearly two weeks.

Abstract Image

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一种新的四芳基AIEgen，用于细胞内脂滴的高选择性成像及其在晚期人类宫颈癌症组织中的检测。

近年来，脂滴引起了人们的广泛关注。它们是细胞的能量库，在许多生理过程中也发挥着重要作用。此外，发现LD与多种病理状况有关，包括癌症和糖尿病。在此，我们报道了一类新的基于四芳基的供体受体结合的聚集诱导发射发光原（AIEgen），6a，用于在体外活的3T3-L1前脂肪细胞和HeLa癌症细胞系中选择性染色细胞内LD。此外，发现AIEgen 6a能够染色和定量晚期人类癌症患者组织切片中的LD积累。与商业LD染色染料尼罗红、BODIPY和LipidTOX不同，AIEgen 6a显示出高的斯托克斯位移（195nm），12.7ns的良好荧光寿命衰减，并且LD染色持续近两周。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Materials Chemistry B MATERIALS SCIENCE, BIOMATERIALS-

CiteScore

11.50

自引率

4.30%

发文量

866

期刊介绍： Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive： Antifouling coatings Biocompatible materials Bioelectronics Bioimaging Biomimetics Biomineralisation Bionics Biosensors Diagnostics Drug delivery Gene delivery Immunobiology Nanomedicine Regenerative medicine & Tissue engineering Scaffolds Soft robotics Stem cells Therapeutic devices