Reducing nitrate and tobacco-specific nitrosamine level in burley tobacco leaves through grafting on flue-cured tobacco rootstock.

IF 2.3 3区 生物学 Q2 PLANT SCIENCES
Plant Direct Pub Date : 2023-10-12 eCollection Date: 2023-10-01 DOI:10.1002/pld3.536
Yuqing Feng, Yuanyuan Zhao, Geng Li, Hongzhi Shi
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引用次数: 0

Abstract

Nitrosation of pyridine alkaloids in tobacco generates tobacco-specific nitrosamines (TSNAs), which are notable toxicants in tobacco products and smoke. Burley tobacco, a chloroplast- and nitrogen (N)-deficient phenotype that accumulates high levels of nitrate-nitrogen (NO3-N) in its leaves, is particularly susceptible to TSNAs formation. In this study, reciprocal pot and field grafting experiments were conducted using burley tobacco Eyan No.1 and flue-cured tobacco K326 to investigate whether grafting burley tobacco scions on flue-cured tobacco rootstocks could enhance pigment biosynthesis and photosynthesis, while reducing the NO3-N level in burley tobacco leaves. Grafting burley tobacco scions on flue-cured tobacco rootstocks significantly increased the total pigment content, photosynthetic rate, biomass, nitrate reductase and glutamine synthetase activities, as well as ammonium-nitrogen (NH4-N), total soluble and reducing sugar, and soluble protein levels in burley tobacco leaves compared with burley tobacco self-rooting, while decreasing the NO3-N level and nitrate-N to total N ratio. Transcriptomic analysis revealed that grafting resulted in upregulated expression of genes involved in starch, sucrose, porphyrin, chlorophyll, and N metabolism, as well as carbon fixation and carotenoid biosynthesis. The findings suggest that grafting on high N use efficiency rootstock is an exceptionally promising means of decreasing NO3-N accumulation by improving photosynthesis and N metabolism in the scion, thereby reducing the levels of harmful TSNAs.

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通过在烤烟砧木上嫁接降低白肋烟叶片中的硝酸盐和烟草特定亚硝胺水平。
烟草中吡啶生物碱的硝化作用会产生烟草特有的亚硝胺(TSNA),这是烟草产品和烟雾中的显著毒物。白肋烟是一种叶绿体和氮(N)缺乏的表型,在其叶片中积累高水平的硝酸盐氮(NO3-N),特别容易形成TSNAs。本研究以艾烟1号和K326号白肋烟为材料,采用盆栽和田间相互嫁接试验,研究了在烤烟砧木上嫁接白肋烟接穗是否能提高色素生物合成和光合作用,同时降低白肋烟叶片中NO3-N水平。与白肋烟自生根相比,在烤烟砧木上嫁接白肋烟接穗显著提高了白肋烟叶片中的总色素含量、光合速率、生物量、硝酸还原酶和谷氨酰胺合成酶活性,以及铵态氮(NH4-N)、总可溶性和还原糖以及可溶性蛋白水平,同时降低NO3-N水平和硝态氮与总氮的比例。转录组学分析显示,嫁接导致淀粉、蔗糖、卟啉、叶绿素和氮代谢以及碳固定和类胡萝卜素生物合成相关基因的表达上调。研究结果表明,在高氮利用效率砧木上嫁接是一种非常有前途的方法,可以通过改善接穗的光合作用和氮代谢来减少NO3-N的积累,从而降低有害的TSNA水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant Direct
Plant Direct Environmental Science-Ecology
CiteScore
5.00
自引率
3.30%
发文量
101
审稿时长
14 weeks
期刊介绍: Plant Direct is a monthly, sound science journal for the plant sciences that gives prompt and equal consideration to papers reporting work dealing with a variety of subjects. Topics include but are not limited to genetics, biochemistry, development, cell biology, biotic stress, abiotic stress, genomics, phenomics, bioinformatics, physiology, molecular biology, and evolution. A collaborative journal launched by the American Society of Plant Biologists, the Society for Experimental Biology and Wiley, Plant Direct publishes papers submitted directly to the journal as well as those referred from a select group of the societies’ journals.
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