The role of MicroRNA as a potential diagnostic and prognostic biomarker for cerebral aneurysms.

IF 1.2 4区医学 Q4 CLINICAL NEUROLOGY

Neurosciences Pub Date : 2023-10-01 DOI:10.17712/nsj.2023.4.20230028

Hosam M Al-Jehani, Alaa N Turkistani, Majd M Alrayes, May A AlHamid, Arwa S AlShamikh, Zhra M Al Sadah, Faisal M Alabbas, Cyril Cyrus, Amein K Al-Ali

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引用次数: 0

Abstract

Objectives: To identify the dysregulated functional miRNAs, miRNA-16, miRNA-143, and miRNA-200 as potential biomarkers of cerebral aneurysms(CAs) to aid in diagnosis and prognosis.

Methods: This is a prospective case-control study conducted among patients with CA. All patients' computed tomography angiography (CTA) and interventional angiogram were assessed and studied. The miRNAs were isolated and quantified from peripheral blood samples and the expression profiling was done using TaqMan chemistry on Real-Time PCR.

Results: A total of 37 samples were included. Three cases had double aneurysms and 10 cases presented with aneurysmal rupture. miRNA-16, miRNA-143, and miRNA-200 were upregulated with an absolute fold-change of >8 in the CA group in comparison to controls (p<0.05). miRNA-200 showed double expression in patients with single aneurysm. A statically significant increase was observed in the expression of miRNA-143 in patients who had an aneurysmal rupture with p<0.05. Diabetic patients showed an obvious increase in miRNA-200 (13.03 folds) and miRNA-16 (26.82 folds) expression. Also, there was a notable elevation in miRNA-16, miRNA-143, and miRNA-200 in patients who had hypertension in comparison to those who did not.

Conclusion: miRNA-16, miRNA-143, and miRNA-200 showed statically significant higher expression among cases with cerebral aneurysms in comparison to controls. Thus, these preliminary results of miRNAs biomarkers are promising future tool to be used for aneurysmal screening.

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微小核糖核酸作为脑动脉瘤潜在诊断和预后生物标志物的作用。

目的：鉴定功能失调的miRNAs、miRNA-16、miRNA-143和miRNA-200作为脑动脉瘤（CA）的潜在生物标志物，以帮助诊断和预后。方法：这是一项在CA患者中进行的前瞻性病例对照研究。对所有患者的计算机断层造影（CTA）和介入血管造影进行评估和研究。从外周血样本中分离和定量miRNA，并在实时PCR上使用TaqMan化学进行表达谱分析。结果：共纳入37个样本。3例为双动脉瘤，10例为动脉瘤破裂。miRNA-16、miRNA-143，与对照组相比，CA组的miRNA-200表达上调，绝对倍数变化>8（pp结论：与对照组比较，miRNA-16、miRNA-143和miRNA-200在脑动脉瘤病例中显示出静态显著的高表达。因此，miRNAs生物标志物的这些初步结果是用于动脉瘤筛查的有前景的未来工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neurosciences 医学-临床神经学

CiteScore

1.40

自引率

0.00%

发文量

审稿时长

4.5 months

期刊介绍： Neurosciences is an open access, peer-reviewed, quarterly publication. Authors are invited to submit for publication articles reporting original work related to the nervous system, e.g., neurology, neurophysiology, neuroradiology, neurosurgery, neurorehabilitation, neurooncology, neuropsychiatry, and neurogenetics, etc. Basic research withclear clinical implications will also be considered. Review articles of current interest and high standard are welcomed for consideration. Prospective workshould not be backdated. There are also sections for Case Reports, Brief Communication, Correspondence, and medical news items. To promote continuous education, training, and learning, we include Clinical Images and MCQ’s. Highlights of international and regional meetings of interest, and specialized supplements will also be considered. All submissions must conform to the Uniform Requirements.