Cir-DNA Sequencing Revealed the Landscape of Extrachromosomal Circular DNA in Articular Cartilage and the Potential Roles in Osteoarthritis.

IF 2.7 4区医学 Q1 ORTHOPEDICS

CARTILAGE Pub Date : 2025-03-01 Epub Date: 2023-10-16 DOI:10.1177/19476035231205690

Wei Xiang, Tongyi Zhang, Song Li, Yunquan Gong, Xiaoqing Luo, Jing Yuan, Yaran Wu, Xiaojing Yan, Yan Xiong, Jiqin Lian, Guangyu Zhao, Changyue Gao, Liang Kuang, Zhenhong Ni

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引用次数: 0

Abstract

Objective: Extrachromosomal circular DNA (eccDNA) has been shown to be involved in several physiological and pathological processes including immunity, inflammation, aging, and tumor. However, the expression of eccDNA in cartilage has not been reported until now. In this study, we aimed to investigate the landscape of eccDNA in articular cartilage and analyze the potential roles in osteoarthritis (OA).

Methods: The samples of articular cartilage were obtained from total knee arthroplasty (TKA) donors with OA. The mitochondrial DNA (mtDNAs) and the linear DNAs from chondrocytes of articular cartilage were removed. Then the eccDNAs were enriched for cir-DNA sequencing. After quality control evaluation, we systematically revealed the identified eccDNA data including size distribution, the size range, and sequence pattern. Moreover, we explored and discussed the potential roles of eccDNA in OA via motif analysis and Gene Ontology (GO)/Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.

Results: The chondrocytes from OA cartilage contained an abundance of eccDNAs, which was termed as OC-eccDNAs (OA cartilage-derived eccDNA). The characteristics of OC-eccDNAs were tissue-specific, including the distribution, the size range, and sequence pattern. Moreover, the functional analysis indicated that eccDNA may be involved in the homeostasis maintenance of chondrocytes and participated in the process of OA.

Conclusions: Our data first showed the landscape of eccDNA in articular cartilage and preliminarily indicated the potential roles of eccDNA in OA.

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Circ-DNA测序揭示了关节软骨中染色体外环状DNA的分布及其在骨关节炎中的潜在作用。

目的：染色体外环状DNA（eccDNA）已被证明参与多种生理和病理过程，包括免疫、炎症、衰老和肿瘤。然而，eccDNA在软骨中的表达直到现在还没有报道。在本研究中，我们旨在研究关节软骨中eccDNA的分布，并分析其在骨关节炎（OA）中的潜在作用。方法：从患有OA的全膝关节置换术（TKA）供体中获取关节软骨样本。从关节软骨细胞中去除线粒体DNA（mtDNA）和线性DNA。然后富集eccDNA进行cir DNA测序。经过质量控制评估，我们系统地揭示了已鉴定的eccDNA数据，包括大小分布、大小范围和序列模式。此外，我们通过基序分析和基因本体论（GO）/京都基因和基因组百科全书（KEGG）通路分析，探索和讨论了eccDNA在OA中的潜在作用。结果：OA软骨细胞中含有丰富的eccDNA，称为OC eccDNA（OA软骨衍生的eccDNAs）。OC eccDNA的特征是组织特异性的，包括分布、大小范围和序列模式。此外，功能分析表明，eccDNA可能参与软骨细胞的稳态维持，并参与OA的形成过程。结论：我们的数据首次显示了关节软骨中eccDNA的分布，并初步表明了eccDNA在OA中的潜在作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

CARTILAGE ORTHOPEDICS-

CiteScore

6.90

自引率

7.10%

发文量

期刊介绍： CARTILAGE publishes articles related to the musculoskeletal system with particular attention to cartilage repair, development, function, degeneration, transplantation, and rehabilitation. The journal is a forum for the exchange of ideas for the many types of researchers and clinicians involved in cartilage biology and repair. A primary objective of CARTILAGE is to foster the cross-fertilization of the findings between clinical and basic sciences throughout the various disciplines involved in cartilage repair. The journal publishes full length original manuscripts on all types of cartilage including articular, nasal, auricular, tracheal/bronchial, and intervertebral disc fibrocartilage. Manuscripts on clinical and laboratory research are welcome. Review articles, editorials, and letters are also encouraged. The ICRS envisages CARTILAGE as a forum for the exchange of knowledge among clinicians, scientists, patients, and researchers. The International Cartilage Repair Society (ICRS) is dedicated to promotion, encouragement, and distribution of fundamental and applied research of cartilage in order to permit a better knowledge of function and dysfunction of articular cartilage and its repair.