Humoral immune response and changes in peritoneal cell populations in rats immunized against two Leptospira serovars; serovar patoc and serovar pyrogenes.

IF 2.9 4区 医学 Q3 IMMUNOLOGY
Dakshika Gangani, Wathsala Dissanayake, Rajiva de Silva, Kaushalya Anuradha, Lilani Karunanayake, Narmada Fernando, Senaka Rajapakse, Sunil Premawansa, Shiroma Handunnetti
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引用次数: 0

Abstract

Background: Leptospirosis is a zoonotic disease caused by Leptospira species. Variations in lipopolysaccharide (LPS) structure in Leptospira are known to be associated with the serovar diversity and antigenicity. Development of immunodiagnostics for early detection of leptospirosis based on immune responses against different pathogenic antigens as well as development of vaccines are important. Hence, this study has assessed the immune response generated against leptospiral LPS and whole antigen preparations of pathogenic and saprophytic Leptospira and specific changes in peritoneal cells was also studied to elucidate the cellular responses associated with immune response of Wistar rats.

Methods: During the study, immune response induced by two types of Leptospira antigen preparations of two selected serovars was compared. Changes in the specific peritoneal cell subpopulations following immunizations of rats were analyzed using flow cytometry.

Results: Of the two antigen preparations tested, the LPS extract induced a higher IgM immune response as opposed to the sonicated antigen preparation. Of the two serovars tested, L. interrogans serovar Pyrogenes had induced a higher IgM response compared to that by L. biflexa serovar Patoc. Considering the IgG titers, equivalent responses were observed with all four antigen preparations. Significant increases in lymphocytes were observed following immunization with LPS of both serovars. Interestingly, the B2 cell percentages increased significantly during the immunization period. Further, significant correlations were observed with both IgM and IgG responses and percentage of B2 cells in the peritoneal cavity (PC).

Conclusion: LPS extract of L. interrogans serovar Pyrogenes induced higher IgM response while the IgG response was equivalent among the four antigen preparations tested. Significant increase of B2 cell percentage in the peritoneal cavity during the immunization reflects the accumulation of B2 cells in the PC which may play considerable role in generating humoral response against Leptospira antigens.

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两种钩端螺旋体血清型免疫大鼠的体液免疫反应和腹膜细胞群的变化;patoc血清型和热原血清型。
背景:钩端螺旋体病是由钩端螺旋菌引起的一种人畜共患疾病。已知钩端螺旋体脂多糖(LPS)结构的变化与血清型多样性和抗原性有关。基于对不同致病抗原的免疫反应开发用于早期检测钩端螺旋体病的免疫诊断以及开发疫苗是重要的。因此,本研究评估了针对致病性和腐生性钩端螺旋体的钩端螺旋LPS和全抗原制剂产生的免疫反应,并研究了腹膜细胞的特异性变化,以阐明与Wistar大鼠免疫反应相关的细胞反应。方法:在研究过程中,比较两种血清型钩端螺旋体抗原制剂诱导的免疫反应。用流式细胞术分析大鼠免疫后腹膜特定细胞亚群的变化。结果:在测试的两种抗原制剂中,与超声处理的抗原制剂相比,LPS提取物诱导了更高的IgM免疫反应。在测试的两种血清变异株中,与双弯乳杆菌血清变异株Patoc相比,询问乳杆菌Pyrogenes血清变异株诱导了更高的IgM反应。考虑到IgG滴度,所有四种抗原制剂均观察到等效反应。在用LPS免疫两种血清型后,观察到淋巴细胞的显著增加。有趣的是,B2细胞百分比在免疫期间显著增加。此外,IgM和IgG反应以及腹腔内B2细胞的百分比也存在显著相关性。免疫过程中腹腔中B2细胞百分比的显著增加反映了B2细胞在PC中的积累,这可能在产生针对钩端螺旋体抗原的体液反应中发挥相当大的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Immunology
BMC Immunology 医学-免疫学
CiteScore
5.50
自引率
0.00%
发文量
54
审稿时长
1 months
期刊介绍: BMC Immunology is an open access journal publishing original peer-reviewed research articles in molecular, cellular, tissue-level, organismal, functional, and developmental aspects of the immune system as well as clinical studies and animal models of human diseases.
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