Green synthesis, characterisation and antibacterial activities of Strobilanthes crispus-mediated silver nanoparticles (SC-AGNPS) against selected bacteria.

IF 4.5 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-10-17 DOI:10.1080/21691401.2023.2268167

Rohazila Mohamad Hanafiah, Siti Aisyah Abd Ghafar, Vuanghao Lim, Siti Nor Asma Musa, Fahmi Yakop, Arif Haikal Hairil Anuar

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Abstract

This study aims to characterize and determine the antibacterial activities of synthesized Strobilanthes crispus-mediated AgNPs (SC-AgNPs) against Streptococcus mutans, Escherichia coli and Pseudomonas aeruginosa. S. crispus water extract acts as a reducing and capping agent in the synthesis of AgNPs. The synthesized AgNPs were characterized by using UV-Vis spectrophotometer, dynamic light scattering (DLS), field emission scanning electron microscope (FESEM), X-ray diffractometer (XRD) and Fourier transform infra-red (FTIR). FESEM images showed a rough surface with a spherical shape. The average size distribution of 75.25 nm with a polydispersity index (PDI) of 0.373. XRD analysis matched the face-centred cubic structure of silver. FTIR analysis revealed a shifted peak from 1404.99 to 1345.00 cm^-1. MIC and MBC values of SC-AgNPs were 1.25 mg/mL and 2.5 mg/mL against E. coli, P. aeruginosa and S. mutans, respectively. Time-kill assay showed that SC-AgNPs significantly reduced bacterial growth as compared to non-treated bacteria. Morphologies of bacteria treated with SC-AgNPs were shrunk, lysed, irregular and smaller as compared to control. SC-AgNPs significantly disrupted the gene expression of eae A, gtf B and Pel A (p < 0.05). This study indicated that the synthesized SC-AgNPs were stable with enhanced antibacterial activities.

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脆叶牛膝介导的银纳米粒子（SC-AGNPS）的绿色合成、表征及其对选定细菌的抗菌活性。

本研究旨在表征和测定合成的脆牛膝介导的AgNPs（SC AgNPs）对变形链球菌、大肠杆菌和铜绿假单胞菌的抗菌活性。薯片水提取物在AgNPs的合成中起到还原和封端剂的作用。利用紫外-可见分光光度计、动态光散射（DLS）、场发射扫描电子显微镜（FESEM）、X射线衍射仪（XRD）和傅里叶变换红外光谱（FTIR）对合成的AgNPs进行了表征。FESEM图像显示粗糙表面呈球形。平均粒度分布为75.25 nm，多分散指数（PDI）为0.373。XRD分析与银的面心立方结构相匹配。FTIR分析显示峰值从1404.99移动到1345.00 cm-1。SC AgNPs的MIC和MBC值为1.25 mg/mL和2.5 mg/mL分别对抗大肠杆菌、铜绿假单胞菌和变形链球菌。时间杀伤试验表明，与未处理的细菌相比，SC AgNPs显著减少了细菌生长。与对照相比，用SC AgNPs处理的细菌的形态缩小、裂解、不规则且更小。SC AgNPs显著破坏eae A、gtf B和Pel A的基因表达（p

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来源期刊

Artificial Cells, Nanomedicine, and Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-ENGINEERING, BIOMEDICAL

CiteScore

10.90

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： Artificial Cells, Nanomedicine and Biotechnology covers the frontiers of interdisciplinary research and application, combining artificial cells, nanotechnology, nanobiotechnology, biotechnology, molecular biology, bioencapsulation, novel carriers, stem cells and tissue engineering. Emphasis is on basic research, applied research, and clinical and industrial applications of the following topics:artificial cellsblood substitutes and oxygen therapeuticsnanotechnology, nanobiotecnology, nanomedicinetissue engineeringstem cellsbioencapsulationmicroencapsulation and nanoencapsulationmicroparticles and nanoparticlesliposomescell therapy and gene therapyenzyme therapydrug delivery systemsbiodegradable and biocompatible polymers for scaffolds and carriersbiosensorsimmobilized enzymes and their usesother biotechnological and nanobiotechnological approachesRapid progress in modern research cannot be carried out in isolation and is based on the combined use of the different novel approaches. The interdisciplinary research involving novel approaches, as discussed above, has revolutionized this field resulting in rapid developments. This journal serves to bring these different, modern and futuristic approaches together for the academic, clinical and industrial communities to allow for even greater developments of this highly interdisciplinary area.