Cytotoxic phenazine and antiallergic phenoxazine alkaloids from an arctic Nocardiopsis dassonvillei SCSIO 502F

IF 4.8 3区化学 Q1 CHEMISTRY, MEDICINAL

Natural Products and Bioprospecting Pub Date : 2023-10-18 DOI:10.1007/s13659-023-00408-w

Yue Song, Qi-Yang Li, Meng-Jing Cong, Xiao-Yan Pang, Bo Chen, Yong-Hong Liu, Li Liao, Jun-Feng Wang

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Abstract

Microbes well-adapted to the Arctic Ocean are promising for producing novel compounds, due to their fancy strategies for adaptation and being under-investigated. Two new phenazine alkaloids (1 and 2) and one new phenoxazine (3) were isolated from Nocardiopsis dassonvillei 502F, a strain originally isolated from Arctic deep-sea sediments. AntiSMASH analysis of the genome of Nocardiopsis dassonvillei 502F revealed the presence of 16 putative biosynthetic gene clusters (BGCs), including a phenazine BGC. Most of the isolated compounds were evaluated for their antibacterial, antiallergic, and cytotoxic activities. Among them, compounds 4 and 5 exhibited potent in vitro cytotoxic activities against osteosarcoma cell line 143B with IC₅₀ values 0.16 and 20.0 μM, respectively. Besides, the results of antiallergic activities of compounds 6–8 exhibited inhibitory activities with IC₅₀ values of 10.88 ± 3.05, 38.88 ± 3.29, and 2.44 ± 0.17 μg/mL, respectively (IC₅₀ 91.6 μM for the positive control loratadine).

Graphical Abstract

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北极诺卡氏病SCSIO502F的细胞毒性吩嗪和抗变态反应吩恶嗪生物碱。

适应北冰洋的微生物很有可能产生新的化合物，因为它们具有奇特的适应策略，而且正在研究中。从北极深海沉积物中分离得到2个新的吩嗪生物碱（1和2）和1个新的苯恶嗪（3）。对Nocardiosis dassonvillei 502F基因组的抗SMASH分析显示存在16个假定的生物合成基因簇（BGCs），包括吩嗪BGCs。大多数分离的化合物都被评估为具有抗菌、抗过敏和细胞毒性活性。其中，化合物4和5对骨肉瘤细胞系143B表现出强大的体外细胞毒性活性，IC50值分别为0.16和20.0μM。此外，化合物6-8的抗变态反应活性结果显示出抑制活性，IC50值为10.88 ± 3.05、38.88 ± 3.29和2.44 ± 0.17μg/mL（阳性对照氯雷他定的IC50为91.6μM）。

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来源期刊

Natural Products and Bioprospecting CHEMISTRY, MEDICINAL-

CiteScore

8.30

自引率

2.10%

发文量

审稿时长

13 weeks

期刊介绍： Natural Products and Bioprospecting serves as an international forum for essential research on natural products and focuses on, but is not limited to, the following aspects: Natural products: isolation and structure elucidation Natural products: synthesis Biological evaluation of biologically active natural products Bioorganic and medicinal chemistry Biosynthesis and microbiological transformation Fermentation and plant tissue cultures Bioprospecting of natural products from natural resources All research articles published in this journal have undergone rigorous peer review. In addition to original research articles, Natural Products and Bioprospecting publishes reviews and short communications, aiming to rapidly disseminate the research results of timely interest, and comprehensive reviews of emerging topics in all the areas of natural products. It is also an open access journal, which provides free access to its articles to anyone, anywhere.