Long-term spaceflight composite stress induces depressive behaviors in model rats through disrupting hippocampus synaptic plasticity

IF 4.8 1区医学 Q1 NEUROSCIENCES

CNS Neuroscience & Therapeutics Pub Date : 2023-10-17 DOI:10.1111/cns.14438

Yi-Shu Yin, Yuan-Bing Zhu, Jun-Lian Liu, Quan-Chun Fan, Xiao-Rui Wu, Shuang Zhao, Jia-Ping Wang, Yu Liu, Yong-Zhi Li, Wei-Hong Lu

{"title":"Long-term spaceflight composite stress induces depressive behaviors in model rats through disrupting hippocampus synaptic plasticity","authors":"Yi-Shu Yin, Yuan-Bing Zhu, Jun-Lian Liu, Quan-Chun Fan, Xiao-Rui Wu, Shuang Zhao, Jia-Ping Wang, Yu Liu, Yong-Zhi Li, Wei-Hong Lu","doi":"10.1111/cns.14438","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Introduction</h3>\n \n <p>Long-term spaceflight composite stress (LSCS) can cause adverse effects on human systems, including the central nervous system, which could trigger anxiety and depression.</p>\n </section>\n \n <section>\n \n <h3> Aims</h3>\n \n <p>This study aimed to identify changes in hippocampus synaptic plasticity under LSCS.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>The present study simulated the real long-term space station environment by conducting a 42-day experiment that involved simulating microgravity, isolation, noise, circadian rhythm disruptions, and low pressure. The mood and behavior of the rats were assessed by behavior test. Transmission electron microscopy and patch-clamp were used to detect the changes in synapse morphology and electrophysiology, and finally, the expression of NMDA receptor channel proteins was detected by western blotting.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The results showed that significant weight loss, anxiety, and depressive behaviors in rats were observed after being exposed to LSCS environment for 42 days. The synaptic structure was severely damaged, manifested as an obvious decrease in postsynaptic density thickness and synaptic interface curvature (<i>p</i> < 0.05; <i>p</i> < 0.05, respectively). Meanwhile, LTP was significantly impaired (<i>p</i> < 0.0001), and currents in the NMDAR channel were also significantly reduced (<i>p</i> < 0.0001). Further analysis found that LSCS decreased the expression of two key subtype proteins on this channel.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>These results suggested that LSCS-induced depressive behaviors by impairing synaptic plasticity in rat hippocampus.</p>\n </section>\n </div>","PeriodicalId":154,"journal":{"name":"CNS Neuroscience & Therapeutics","volume":"30 3","pages":""},"PeriodicalIF":4.8000,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cns.14438","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"CNS Neuroscience & Therapeutics","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/cns.14438","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"NEUROSCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction

Long-term spaceflight composite stress (LSCS) can cause adverse effects on human systems, including the central nervous system, which could trigger anxiety and depression.

Aims

This study aimed to identify changes in hippocampus synaptic plasticity under LSCS.

Methods

The present study simulated the real long-term space station environment by conducting a 42-day experiment that involved simulating microgravity, isolation, noise, circadian rhythm disruptions, and low pressure. The mood and behavior of the rats were assessed by behavior test. Transmission electron microscopy and patch-clamp were used to detect the changes in synapse morphology and electrophysiology, and finally, the expression of NMDA receptor channel proteins was detected by western blotting.

Results

The results showed that significant weight loss, anxiety, and depressive behaviors in rats were observed after being exposed to LSCS environment for 42 days. The synaptic structure was severely damaged, manifested as an obvious decrease in postsynaptic density thickness and synaptic interface curvature (p < 0.05; p < 0.05, respectively). Meanwhile, LTP was significantly impaired (p < 0.0001), and currents in the NMDAR channel were also significantly reduced (p < 0.0001). Further analysis found that LSCS decreased the expression of two key subtype proteins on this channel.

Conclusion

These results suggested that LSCS-induced depressive behaviors by impairing synaptic plasticity in rat hippocampus.

Abstract Image

查看原文本刊更多论文

长期航天复合应激通过破坏海马突触可塑性诱导模型大鼠抑郁行为。

引言：长期航天复合应激（LSCS）会对包括中枢神经系统在内的人体系统造成不良影响，从而引发焦虑和抑郁。目的：本研究旨在确定LSCS下海马突触可塑性的变化。方法：本研究通过进行为期42天的实验模拟了真实的长期空间站环境，包括模拟微重力、隔离、噪音、昼夜节律紊乱和低压。通过行为测试来评估大鼠的情绪和行为。用透射电镜和膜片钳检测突触形态和电生理的变化，最后用蛋白质印迹法检测NMDA受体通道蛋白的表达。结果：大鼠暴露于LSCS环境42小时后，体重显著减轻，出现焦虑和抑郁行为天。突触结构严重受损，表现为突触后密度厚度和突触界面曲率明显降低（p 结论：LSCS通过损害大鼠海马突触可塑性而诱发抑郁行为。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

CNS Neuroscience & Therapeutics 医学-神经科学

CiteScore

7.30

自引率

12.70%

发文量

240

审稿时长

2 months

期刊介绍： CNS Neuroscience & Therapeutics provides a medium for rapid publication of original clinical, experimental, and translational research papers, timely reviews and reports of novel findings of therapeutic relevance to the central nervous system, as well as papers related to clinical pharmacology, drug development and novel methodologies for drug evaluation. The journal focuses on neurological and psychiatric diseases such as stroke, Parkinson’s disease, Alzheimer’s disease, depression, schizophrenia, epilepsy, and drug abuse.