Methods and applications of CRISPR/Cas system for genome editing in stem cells

IF 4 Q2 CELL & TISSUE ENGINEERING

Cell Regeneration Pub Date : 2019-12-01 DOI:10.1016/j.cr.2019.08.001

Guang Yang, Xingxu Huang

引用次数: 24

Abstract

Genome editing technology holds great promise for genome manipulation and gene therapy. While widespread utilization, genome editing has been used to unravel the roles of specific genes in differentiation and pluripotency of stem cells, and reinforce the stem cell-based applications. In this review, we summarize the advances of genome editing technology, as well as the derivative technologies from CRISPR/Cas system, which show tremendous potential in various fields. We also highlight the key findings in the studies of stem cells and regeneration by genome editing technology.

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CRISPR/Cas系统在干细胞基因组编辑中的方法及应用

基因组编辑技术为基因组操作和基因治疗带来了巨大的希望。在广泛应用的同时，基因组编辑已被用于揭示特定基因在干细胞分化和多能性中的作用，并加强基于干细胞的应用。本文综述了基因组编辑技术的进展，以及CRISPR/Cas系统衍生技术，这些技术在各个领域显示出巨大的潜力。我们还重点介绍了基因组编辑技术在干细胞和再生研究中的关键发现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Regeneration Biochemistry, Genetics and Molecular Biology-Cell Biology

CiteScore

5.80

自引率

0.00%

发文量

审稿时长

35 days

期刊介绍： Cell Regeneration aims to provide a worldwide platform for researches on stem cells and regenerative biology to develop basic science and to foster its clinical translation in medicine. Cell Regeneration welcomes reports on novel discoveries, theories, methods, technologies, and products in the field of stem cells and regenerative research, the journal is interested, but not limited to the following topics: ◎ Embryonic stem cells ◎ Induced pluripotent stem cells ◎ Tissue-specific stem cells ◎ Tissue or organ regeneration ◎ Methodology ◎ Biomaterials and regeneration ◎ Clinical translation or application in medicine