Multispectral Imaging of Metabolic Fluorophores: Comparing In Vivo and Fresh Ex Vivo Tissue.

IF 1.5 4区 医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Gary E Carver, Sarah A Locknar, Prachi N Ghule, Christopher J Pung, Donald L Weaver, Janet L Stein, Gary S Stein
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引用次数: 0

Abstract

The enhanced uptake of glucose by cancer cells via aerobic glycolysis occurs when the lactic acid pathway is favored over the citric acid cycle. The lactic acid cycle in cancer cells influences the cytosolic concentration of metabolic fluorophores including NADH (the reduced form of nicotinamide adenine dinucleotide) and flavin adenine dinucleotide (FAD). In particular, the literature has shown that breast cancer influences the relative magnitude of fluorescence from NADH and FAD. A multispectral imaging system has been developed for rapid non-destructive imaging of intrinsic fluorescence in tissue. This paper compares in vivo data to fresh ex vivo data gathered as a function of time in mouse models. The data indicate that, if measured within 30 min of excision, a cancer diagnosis in fresh ex vivo tissue correlates with a cancer diagnosis in in vivo tissue. These results justify a plan to evaluate fresh ex vivo human tissue to quantify the sensitivity and specificity of the multispectral system.

代谢荧光团的多光谱成像:体内和新鲜离体组织的比较。
当乳酸途径优于柠檬酸循环时,癌症细胞通过有氧糖酵解对葡萄糖的吸收增强。癌症细胞中的乳酸循环影响包括NADH(烟酰胺腺嘌呤二核苷酸的还原形式)和黄素腺嘌呤二核苷酸(FAD)在内的代谢荧光团的胞浆浓度。特别是,文献表明,癌症影响NADH和FAD荧光的相对大小。已经开发了一种用于组织中固有荧光的快速无损成像的多光谱成像系统。本文比较了在小鼠模型中作为时间函数收集的体内数据和新鲜的离体数据。数据表明,如果在切除后30分钟内测量,新鲜离体组织中的癌症诊断与体内组织中的癌症诊断相关。这些结果证明了评估新鲜离体人体组织以量化多光谱系统的敏感性和特异性的计划是合理的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Critical Reviews in Eukaryotic Gene Expression
Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物
CiteScore
2.70
自引率
0.00%
发文量
67
审稿时长
1 months
期刊介绍: Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.
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