Analysis of Transgenic Cotton Plants Containing Universal Stress Protein (GaUSP-1, GaUSP-2) and Zinc Finger Transcriptional Factor (GaZnF) Genes under Drought Stress.

IF 1.5 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-01-01 DOI:10.1615/CritRevEukaryotGeneExpr.2023048905

Muhammad Hamza Basit, Bushra Rasheed, Kausar Malik, Zoha Masood, Samia Afzal

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引用次数: 0

Abstract

Water is the most limiting factor for plant growth and crop productivity. Drought stress adversely affects crop yield throughout the world. Up to 50% of crop yield in Pakistan is severely affected by the shortage of water. Cotton is an important cash crop for Pakistan known as "white gold." It accounts for 8.2% of the value added in agriculture and about 3.2% of GDP. Besides, being the world's fourth-largest cotton producer, our yield per acre ranks 13th in the world. If we look at the Pakistan scenario, water deficiency is one of the major yield-limiting factors. Limitations related to conventional breeding and the advancements in plant genomics and biotechnology applications have opened new horizons to plant improvements. Therefore, in the current study, we carry out a comparative analysis to evaluate the morphological, physiological biochemical and molecular parameters in transgenic plants containing GaUSP-1, GaUSP-2 and GaZinc Finger genes under different drought stress conditions. Data showed that transgenic plants showed more tolerance as compared to non-transgenic plants. Transgenic and non-transgenic assist us in our better understanding of the drought-responsive mechanism and its effect on different plant growth traits, so, in this way, we would be able to explore drought tolerance mechanism and this will open the doors for the identification of drought-related genes.

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干旱胁迫下含通用胁迫蛋白（GaUSP-1，GaUSP-2）和锌指转录因子（GaZnF）基因的转基因棉花植株的分析。

水是限制植物生长和作物生产力的最重要因素。干旱胁迫对世界各地的作物产量产生不利影响。巴基斯坦高达50%的作物产量受到缺水的严重影响。棉花是巴基斯坦重要的经济作物，被称为“白金”。棉花占农业增加值的8.2%，约占国内生产总值的3.2%。此外，作为世界第四大棉花生产国，我们的亩产量位居世界第13位。如果我们看看巴基斯坦的情况，缺水是限制产量的主要因素之一。传统育种的局限性以及植物基因组学和生物技术应用的进步为植物改良开辟了新的视野。因此，在本研究中，我们对含有GaUSP-1、GaUSP-2和GaZinc Finger基因的转基因植物在不同干旱胁迫条件下的形态、生理生化和分子参数进行了比较分析。数据显示，与非转基因植物相比，转基因植物表现出更强的耐受性。转基因和非转基因有助于我们更好地了解干旱反应机制及其对不同植物生长性状的影响，因此，通过这种方式，我们将能够探索耐旱机制，这将为鉴定干旱相关基因打开大门。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.