Cytokine expression of IL-8 before and after root canal treatment in patients with symptomatic apical periodontitis: A clinical trial

IF 1.3 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Australian Endodontic Journal Pub Date : 2023-10-11 DOI:10.1111/aej.12803

Sarah Samir Abouelenien MSc, PhD, Olfat Gamil Shaker MSc, PhD, Dina Ahmed Morsy MSc, PhD

{"title":"Cytokine expression of IL-8 before and after root canal treatment in patients with symptomatic apical periodontitis: A clinical trial","authors":"Sarah Samir Abouelenien MSc, PhD, Olfat Gamil Shaker MSc, PhD, Dina Ahmed Morsy MSc, PhD","doi":"10.1111/aej.12803","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <p>This study's objective was to compare the cytokine expression of IL-8 in periapical tissues of single-rooted teeth with symptomatic apical periodontitis (SAP) before and after root canal treatments. As well as, comparing IL-8 levels in peri-apical tissues between vital and necrotic teeth with SAP.</p>\n </section>\n \n <section>\n \n <h3> Methodology</h3>\n \n <p>Thirty-six patients were allocated according to their pulp status into two experimental groups (<i>n</i> = 18) receiving the same treatment protocol; group 1: Vital pulps with SAP, and group 2: non-vital pulps with SAP. Conventional endodontic treatment was done on two visits; isolation and disinfection of the operative field were undertaken, and two-stage access cavity preparation was implemented. The first pre-instrumentation peri-apical sample (S1) was collected prior to cleaning and shaping procedures. A 2.5% NaOCl irrigation was used to thoroughly irrigate the canal after performing root canal preparation utilising the ProTaper Next (PTN) rotary system. After 1 week, the second post-instrumentation peri-apical sample (S2) was collected. Using an ELISA kit, the quantity of IL-8 was evaluated following the collection of all samples.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>In all pre-instrumentation samples, IL-8 was detected (100%). The level of IL-8 expression was significantly decreased from the S1 to S2 of all samples (<i>p</i> < 0.001). The intra-group comparison showed a statistically significant reduction in the level of IL-8 expression between S1 and S2 in both vital and non-vital groups where <i>p</i> < 0.001* in both groups. The inter-group comparison of levels of IL-8 expression (vital and non-vital) revealed a significant difference between both groups regarding the pretreatment sample with the higher levels of IL-8 shown in the non-vital group (<i>p</i> < 0.001). While in the post-treatment sample, both groups showed a significant reduction in the level of IL-8 expression but the difference between them was not statistically significant (<i>p</i> = 0.226).</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Root canal instrumentation seems to be efficient in decreasing the levels of anti-inflammatory cytokines, namely IL-8. Further research should clarify how intra-canal medicaments affect inflammatory mediator levels.</p>\n </section>\n </div>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Australian Endodontic Journal","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/aej.12803","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

This study's objective was to compare the cytokine expression of IL-8 in periapical tissues of single-rooted teeth with symptomatic apical periodontitis (SAP) before and after root canal treatments. As well as, comparing IL-8 levels in peri-apical tissues between vital and necrotic teeth with SAP.

Methodology

Thirty-six patients were allocated according to their pulp status into two experimental groups (n = 18) receiving the same treatment protocol; group 1: Vital pulps with SAP, and group 2: non-vital pulps with SAP. Conventional endodontic treatment was done on two visits; isolation and disinfection of the operative field were undertaken, and two-stage access cavity preparation was implemented. The first pre-instrumentation peri-apical sample (S1) was collected prior to cleaning and shaping procedures. A 2.5% NaOCl irrigation was used to thoroughly irrigate the canal after performing root canal preparation utilising the ProTaper Next (PTN) rotary system. After 1 week, the second post-instrumentation peri-apical sample (S2) was collected. Using an ELISA kit, the quantity of IL-8 was evaluated following the collection of all samples.

Results

In all pre-instrumentation samples, IL-8 was detected (100%). The level of IL-8 expression was significantly decreased from the S1 to S2 of all samples (p < 0.001). The intra-group comparison showed a statistically significant reduction in the level of IL-8 expression between S1 and S2 in both vital and non-vital groups where p < 0.001* in both groups. The inter-group comparison of levels of IL-8 expression (vital and non-vital) revealed a significant difference between both groups regarding the pretreatment sample with the higher levels of IL-8 shown in the non-vital group (p < 0.001). While in the post-treatment sample, both groups showed a significant reduction in the level of IL-8 expression but the difference between them was not statistically significant (p = 0.226).

Conclusion

Root canal instrumentation seems to be efficient in decreasing the levels of anti-inflammatory cytokines, namely IL-8. Further research should clarify how intra-canal medicaments affect inflammatory mediator levels.

查看原文本刊更多论文

症状性根尖周炎患者根管治疗前后IL-8的细胞因子表达：一项临床试验。

本研究的目的是比较根管治疗前后单根牙和症状性根尖周炎（SAP）根尖组织中IL-8的细胞因子表达。方法：36例患者根据牙髓状态分为两组（n = 18）接收相同的治疗方案；第1组：SAP的重要牙髓，第2组：SAP非重要牙髓。常规根管治疗在两次就诊中进行；对手术区进行了隔离和消毒，并进行了两阶段的进入腔准备。在清洁和整形程序之前采集第一个器械植入前根尖周样本（S1）。在使用ProTaper Next（PTN）旋转系统进行根管预备后，使用2.5%NaOCl灌溉来彻底灌溉根管。1之后第二周，采集器械植入后的根尖周样本（S2）。使用ELISA试剂盒，在收集所有样品后评估IL-8的量。结果：在所有仪器前样品中，均检测到IL-8（100%）。所有样品的IL-8表达水平从S1到S2都显著降低（p 结论：根管内固定治疗可有效降低抗炎细胞因子IL-8的水平。进一步的研究应该阐明管内药物如何影响炎症介质水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Australian Endodontic Journal DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.50

自引率

6.20%

发文量

审稿时长

>12 weeks

期刊介绍： The Australian Endodontic Journal provides a forum for communication in the different fields that encompass endodontics for all specialists and dentists with an interest in the morphology, physiology, and pathology of the human tooth, in particular the dental pulp, root and peri-radicular tissues. The Journal features regular clinical updates, research reports and case reports from authors worldwide, and also publishes meeting abstracts, society news and historical endodontic glimpses. The Australian Endodontic Journal is a publication for dentists in general and specialist practice devoted solely to endodontics. It aims to promote communication in the different fields that encompass endodontics for those dentists who have a special interest in endodontics.