GYRB - POLYMERASE CHAIN REACTION AND HISTOPATHOLOGIC CHARACTERISTIC FIGURE POTENTIAL FOR DETERMINING DIAGNOSIS OF TUBERCULOUS LYMPHADENITIS.

Q4 Medicine
African Journal of Infectious Diseases Pub Date : 2023-08-01 eCollection Date: 2023-01-01 DOI:10.21010/Ajidv17i2S.3
Herlambang Wahyu, Mertaniasih Ni Made, Soedarsono Soedarsono, Sandhika Willy
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引用次数: 0

Abstract

Background: TB lymphadenitis is still a problem that needs serious treatment. In Indonesia, it was reported that 53% of TB cases were extrapulmonary tuberculosis, with the most cases being Lymphadenitis TB, 11.6%. In children, 43% of extrapulmonary tuberculosis cases are TB lymphadenitis. Diagnosis is quite difficult; a method of determining the diagnosis and appropriate comprehensive treatment is required in managing TB Lymphadenitis.

Materials and methods: In this study, 15 fine needle aspiration biopsy aspirate samples were subjected to molecular examination using the gyrB-polymerase chain reaction method and histopathological observations using the smear method with hematoxylin-eosin staining. Observation of preparations using a microscope with a magnification of 200x.

Results: The histopathological characteristics of the fine needle aspiration biopsy aspirate showed positive results in 4 out of 15 samples, with epithelioid cells arranged in a characteristic granuloma structure, necrotic debris was visible, and cells joined together to form multinucleated giant cells as an inflammatory response to Mycobacterium tuberculosis complex infection. In this study, 6 out of 15 (40%) were detected to be positive in the diagnosis based on molecular detection using a specific target gene gyrB - polymerase chain reaction .

Conclusion: Characteristic features on histopathological examination associated with gyrB - positive polymerase chain reaction on lymphadenitis fine needle aspiration biopsy aspirate samples can be used as a determinant diagnosis of tuberculous lymphadenitis.

Abstract Image

Abstract Image

GYRB-聚合酶链反应和组织病理学特征图对确定结核性淋巴结炎诊断的潜力。
背景:结核淋巴结炎仍然是一个需要认真治疗的问题。据报道,在印度尼西亚,53%的肺结核病例是肺外结核,其中淋巴结炎结核病例最多,占11.6%。在儿童中,43%的肺外结核病例是结核淋巴结炎。诊断相当困难;需要一种确定诊断和适当综合治疗的方法来管理结核淋巴结炎。材料和方法:本研究采用gyrB聚合酶链反应法对15份细针穿刺活检抽吸标本进行分子检查,并采用苏木精-伊红染色涂片法进行组织病理学观察。使用放大倍数为200倍的显微镜观察制剂。结果:15个样本中有4个样本的细针抽吸活检抽吸物的组织病理学特征显示阳性,上皮样细胞排列成特征性肉芽肿结构,可见坏死碎片,细胞结合在一起形成多核巨细胞,作为对结核分枝杆菌复合感染的炎症反应。在这项研究中,15人中有6人(40%)在使用特定靶基因gyrB-聚合酶链式反应进行分子检测的诊断中被检测为阳性。结论:淋巴结炎细针穿刺活检标本的组织病理学特征与gyrB阳性聚合酶链反应相关,可作为结核性淋巴结炎的决定性诊断。
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来源期刊
African Journal of Infectious Diseases
African Journal of Infectious Diseases Medicine-Infectious Diseases
CiteScore
1.60
自引率
0.00%
发文量
32
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