Yueomyces silvicola sp. nov., a novel ascomycetous yeast species unable to utilize ammonium, glutamate, and glutamine as sole nitrogen sources.

IF 2.2 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yeast Pub Date : 2023-11-01 Epub Date: 2023-10-11 DOI:10.1002/yea.3901
Hong-Tao Yu, Yu-Jie Shang, Hai-Yan Zhu, Pei-Jie Han, Qi-Ming Wang, Ana Raquel O Santos, Katharina O Barros, Gisele F L Souza, Flávia B M Alvarenga, Maxwel A Abegg, Carlos A Rosa, Feng-Yan Bai
{"title":"Yueomyces silvicola sp. nov., a novel ascomycetous yeast species unable to utilize ammonium, glutamate, and glutamine as sole nitrogen sources.","authors":"Hong-Tao Yu,&nbsp;Yu-Jie Shang,&nbsp;Hai-Yan Zhu,&nbsp;Pei-Jie Han,&nbsp;Qi-Ming Wang,&nbsp;Ana Raquel O Santos,&nbsp;Katharina O Barros,&nbsp;Gisele F L Souza,&nbsp;Flávia B M Alvarenga,&nbsp;Maxwel A Abegg,&nbsp;Carlos A Rosa,&nbsp;Feng-Yan Bai","doi":"10.1002/yea.3901","DOIUrl":null,"url":null,"abstract":"<p><p>Five yeast strains isolated from tree bark and rotten wood collected in central and southwestern China, together with four Brazilian strains (three from soil and rotting wood collected in an Amazonian rainforest biome and one from Bromeliad collected in Alagoas state) and one Costa Rican strain isolated from a flower beetle, represent a new species closely related with Yueomyces sinensis in Saccharomycetaceae, as revealed by the 26S ribosomal RNA gene D1/D2 domain and the internal transcribed spacer region sequence analysis. The name Yueomyces silvicola sp. nov. is proposed for this new species with the holotype China General Microbiological Culture Collection Center 2.6469 (= Japan Collection of Microorganisms 34885). The new species exhibits a whole-genome average nucleotide identity value of 77.8% with Y. sinensis. The two Yueomyces species shared unique physiological characteristics of being unable to utilize ammonium and the majority of the amino acids, including glutamate and glutamine, as sole nitrogen sources. Among the 20 amino acids tested, only leucine and tyrosine can be utilized by the Yueomyces species. Genome sequence comparison showed that GAT1, which encodes a GATA family protein participating in transcriptional activation of nitrogen-catabolic genes in Saccharomyces cerevisiae, is absent in the Yueomyces species. However, the failure of the Yueomyces species to utilize ammonium, glutamate, and glutamine, which are generally preferred nitrogen sources for microorganisms, implies that more complicated alterations in the central nitrogen metabolism pathway might occur in the genus Yueomyces.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Yeast","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/yea.3901","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/10/11 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 1

Abstract

Five yeast strains isolated from tree bark and rotten wood collected in central and southwestern China, together with four Brazilian strains (three from soil and rotting wood collected in an Amazonian rainforest biome and one from Bromeliad collected in Alagoas state) and one Costa Rican strain isolated from a flower beetle, represent a new species closely related with Yueomyces sinensis in Saccharomycetaceae, as revealed by the 26S ribosomal RNA gene D1/D2 domain and the internal transcribed spacer region sequence analysis. The name Yueomyces silvicola sp. nov. is proposed for this new species with the holotype China General Microbiological Culture Collection Center 2.6469 (= Japan Collection of Microorganisms 34885). The new species exhibits a whole-genome average nucleotide identity value of 77.8% with Y. sinensis. The two Yueomyces species shared unique physiological characteristics of being unable to utilize ammonium and the majority of the amino acids, including glutamate and glutamine, as sole nitrogen sources. Among the 20 amino acids tested, only leucine and tyrosine can be utilized by the Yueomyces species. Genome sequence comparison showed that GAT1, which encodes a GATA family protein participating in transcriptional activation of nitrogen-catabolic genes in Saccharomyces cerevisiae, is absent in the Yueomyces species. However, the failure of the Yueomyces species to utilize ammonium, glutamate, and glutamine, which are generally preferred nitrogen sources for microorganisms, implies that more complicated alterations in the central nitrogen metabolism pathway might occur in the genus Yueomyces.

Abstract Image

silvicola Yuomyces sp.nov.,一种新的子囊菌酵母,不能利用铵、谷氨酸和谷氨酰胺作为唯一的氮源。
从中国中部和西南部采集的树皮和腐烂木材中分离出的五株酵母菌株,以及四株巴西菌株(三株来自亚马逊雨林生物群落中采集的土壤和腐烂木材,一株来自阿拉戈斯州采集的凤梨属)和一株哥斯达黎加菌株,通过26S核糖体RNA基因D1/D2结构域和内部转录间隔区序列分析,代表了一个与酵母科中华酵母密切相关的新种。该新种经中国微生物总培养物保藏中心正模鉴定,命名为Yuomyces silvicola sp.nov(= 日本微生物保藏中心34885)。该新种与中华绒螯蟹的全基因组平均核苷酸同源性为77.8%。这两种酵母具有独特的生理特征,不能利用铵和大多数氨基酸,包括谷氨酸和谷氨酰胺,作为唯一的氮源。在测试的20种氨基酸中,只有亮氨酸和酪氨酸可以被酵母利用。基因组序列比较表明,GAT1编码一种参与酿酒酵母氮分解代谢基因转录激活的GATA家族蛋白,但在酵母中不存在。然而,酵母属物种不能利用铵、谷氨酸和谷氨酰胺,这通常是微生物首选的氮源,这意味着在酵母属中可能会发生更复杂的中央氮代谢途径改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Yeast
Yeast 生物-生化与分子生物学
CiteScore
5.30
自引率
3.80%
发文量
55
审稿时长
3 months
期刊介绍: Yeast publishes original articles and reviews on the most significant developments of research with unicellular fungi, including innovative methods of broad applicability. It is essential reading for those wishing to keep up to date with this rapidly moving field of yeast biology. Topics covered include: biochemistry and molecular biology; biodiversity and taxonomy; biotechnology; cell and developmental biology; ecology and evolution; genetics and genomics; metabolism and physiology; pathobiology; synthetic and systems biology; tools and resources
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信