Halapricum hydrolyticum sp. nov., a beta-1,3-glucan utilizing haloarchaeon from hypersaline lakes

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Dimitry Y. Sorokin , Alexander G. Elcheninov , Alexander Y. Merkel , Nicole J. Bale , Jaap Sininghe-Damste , Ilya V. Kublanov
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Abstract

Two strains of neutrophilic haloaloarchaea were selectively enriched from hypersaline lakes in southwestern Siberia using β-1,3-glucans as a substrate. The strains were nearly identical in their phenotypes and according to phylogenomic analysis, and represent a distant novel species group in the genus Halapricum of the family Haloarculaceae. The main phenotypic property of the novel isolates is the ability to hydrolyze and grow with the polysaccharides curdlan and pachyman. Such potential has, to date, not been seen in any other haloarchaea in pure cultures. The strains are obligately aerobic saccharolytics. Apart from the insoluble β-1,3-glucans, they utilized soluble α-glucans (starch, pullulan and glycogen) and a limited number of sugars. The major ether-bound polar phospholipids include PGP-Me and PG. The glyco- and sulfolipids were absent. The major respiratory menaquinone is MK-8:8. On the basis of their unique physiological properties and the results of phylogenomic analysis, the isolates are suggested to be classified into a novel species Halapricum hydrolyticum sp. nov. (type strain HArc-curdl5-1T = DSM 114193T = UQM 41587T).

Halapricum hydrolyticum sp.nov.,一种利用高盐度湖泊中卤古菌素的β-1,3-葡聚糖。
以β-1,3-葡聚糖为底物,从西伯利亚西南部的高盐度湖泊中选择性富集了两株嗜中性卤代古菌。根据系统发育学分析,这两个菌株在表型和系统发育学上几乎相同,代表了Haloarculaceae科Halapricum属的一个遥远的新物种群。新分离株的主要表型特征是能够水解多糖凝乳蛋白酶和茯苓多糖并与之一起生长。迄今为止,在纯培养的任何其他卤代古菌中都没有发现这种潜力。这些菌株是专性好氧解糖菌。除了不溶性β-1,3-葡聚糖外,它们还利用可溶性α-葡聚糖(淀粉、支链淀粉和糖原)和有限数量的糖。主要的醚结合极性磷脂包括PGP-Me和PG。不存在糖脂和磺基脂质。呼吸系统中主要的甲萘醌是MK-8:8。根据其独特的生理特性和系统发育组学分析结果,建议将其分为一个新种Halapricum hydrolyticum sp.nov.(型菌株HArc-curdl5-1T=DSM 114193T=UQM 41587T)。
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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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