Thermodynamics and kinetic analysis of carbon nanofibers as nanozymes.

IF 4.9 Q2 NANOSCIENCE & NANOTECHNOLOGY
Nanotechnology, Science and Applications Pub Date : 2019-07-16 eCollection Date: 2019-01-01 DOI:10.2147/NSA.S208310
Maziar Bahreini, Monireh Movahedi, Maryam Peyvandi, Fereshteh Nematollahi, Hessam Sepasi Tehrani
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引用次数: 7

Abstract

Purpose: Evaluation of structural features, thermodynamics and kinetic properties of carbon nanofibers (CNFs) as artificial nanoscale enzymes (nanozyme).

Methods: Synthesis of CNFs was done using chemical vapor deposition, and transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM) and energy-dispersive x-ray spectroscopy (EDX) were used to provide information on the morphology, elemental monitoring and impurity assay of the CNFs. The thermal features of the CNFs were evaluated using differential thermal analysis (DTA), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) derivative and TGA. The calculated thermo-physical parameters were melting temperature (Tm), weight loss maximum temperature (Tmax ) and enthalpy of fusion (ΔHfusion ). Catalytic activity was assayed by a 4-aminoantypyrine (4-AAP)-H2O2 coupled colorimetric system by UV-visible spectroscopy.

Results: FE-SEM and TEM analysis demonstrated parallel graphitic layers and uniformity of atomic orientation and morphology. The EDX spectra approved carbon element as major signal and presence of partial Ti as impurities of CNFs during CVD process. The DTA thermogram showed the endothermic process had a maximum temperature of 82.27°C at -15.48 mV and that thermal decomposition occurred at about 200°C. The TGA-differential gravimetric analysis thermogram showed that Tmax was 700°C. The DSC heat flow curve showed a melting temperature (Tm) of 254.52°C, ΔHfusion of 3.84 J^.g-1, area under the curve of 58.58 mJ and Te (onset) and Tf (end set) temperatures of 246.60°C and 285.67°C, respectively. The peroxidase activity of the CNFs obeyed the Michaelis-Menten equation with a double-reciprocal curve and the calculated Km, Kcat and Vmax kinetic parameters.

Conclusion: CNFs as peroxidase nanozymes are intrinsically strong and stable nanocatalysts under difficult thermal conditions. The peroxidase activity was demonstrated, making these CNFs candidates for analytical tools under extreme conditions.

碳纳米纤维作为纳米酶的热力学和动力学分析。
目的:评价碳纳米纤维(CNFs)作为人工纳米酶(nanozyme)的结构特征、热力学和动力学性能。方法:采用化学气相沉积和透射电子显微镜(TEM)合成CNFs,利用场发射扫描电子显微镜(FE-SEM)和能谱仪(EDX)对CNFs的形貌、元素监测和杂质分析进行了研究。使用差热分析(DTA)、差示扫描量热法(DSC)、热重分析(TGA)衍生物和TGA对CNFs的热特性进行了评估。计算的热物理参数为熔融温度(Tm)、失重最高温度(Tmax)和熔融焓(ΔHfusion)。采用4-氨基安替吡啉(4-AAP)-H2O2偶联比色体系,用紫外-可见光谱法测定催化活性。结果:FE-SEM和TEM分析表明,石墨层平行,原子取向和形貌均匀。EDX光谱证实碳元素是CVD过程中的主要信号,部分Ti作为CNFs的杂质存在。DTA热谱图显示,吸热过程在-15.48mV时的最高温度为82.27°C,热分解发生在约200°C。TGA差示重量分析热谱图显示Tmax为700°C。DSC热流曲线显示熔融温度(Tm)为254.52°C,ΔHfusion为3.84 J^.g-1,曲线下面积为58.58 mJ,Te(起始)和Tf(终凝)温度分别为246.60°C和285.67°C。CNFs的过氧化物酶活性服从具有双倒数曲线的Michaelis-Menten方程以及计算的Km、Kcat和Vmax动力学参数。结论:CNFs作为过氧化物酶纳米酶,在高温条件下是一种本质上强而稳定的纳米催化剂。过氧化物酶活性得到了证明,使这些CNFs成为极端条件下分析工具的候选者。
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来源期刊
Nanotechnology, Science and Applications
Nanotechnology, Science and Applications NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
11.70
自引率
0.00%
发文量
3
审稿时长
16 weeks
期刊介绍: Nanotechnology, Science and Applications is an international, peer-reviewed, Open Access journal that focuses on the science of nanotechnology in a wide range of industrial and academic applications. The journal is characterized by the rapid reporting of reviews, original research, and application studies across all sectors, including engineering, optics, bio-medicine, cosmetics, textiles, resource sustainability and science. Applied research into nano-materials, particles, nano-structures and fabrication, diagnostics and analytics, drug delivery and toxicology constitute the primary direction of the journal.
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