Heterogeneous landscape promotes distinct microbial communities in an imperiled scrub ecosystem.

IF 2.6 2区 生物学 Q2 MYCOLOGY
Mycologia Pub Date : 2023-11-01 Epub Date: 2023-11-14 DOI:10.1080/00275514.2023.2258268
Aaron S David, Damian J Hernandez, Eric S Menges, Vivienne L Sclater, Michelle E Afkhami, Christopher A Searcy
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引用次数: 0

Abstract

Habitat heterogeneity is a key driver of biodiversity of macroorganisms, yet how heterogeneity structures belowground microbial communities is not well understood. Importantly, belowground microbial communities may respond to any number of abiotic, biotic, and spatial drivers found in heterogeneous environments. Here, we examine potential drivers of prokaryotic and fungal communities in soils across the heterogenous landscape of the imperiled Florida scrub, a pyrogenic ecosystem where slight differences in elevation lead to large changes in water and nutrient availability and vegetation composition. We employ a comprehensive, large-scale sampling design to characterize the communities of prokaryotes and fungi associated with three habitat types and two soil depths (crust and subterranean) to evaluate (i) differences in microbial communities across these heterogeneous habitats, (ii) the relative roles of abiotic, biotic, and spatial drivers in shaping community structure, and (iii) the distribution of fungal guilds across these habitats. We sequenced soils from 40 complete replicates of habitat × soil depth combinations and sequenced the prokaryotic 16S and fungal internal transcribed spacer (ITS) regions using Illumina MiSeq. Habitat heterogeneity generated distinct communities of soil prokaryotes and fungi. Spatial distance played a role in structuring crust communities, whereas subterranean microbial communities were primarily structured by the shrub community, whose roots they presumably interacted with. This result helps to explain the unexpected transition we observed between arbuscular mycorrhiza-dominated soils at low-elevation habitats to ectomycorrhiza-dominated soils at high-elevation habitats. Our results challenge previous notions of environmental determinism of microbial communities and generate new hypotheses regarding symbiotic relationships across heterogeneous environments.

异质景观促进了濒危灌木生态系统中不同的微生物群落。
生境异质性是大型生物多样性的关键驱动因素,但地下微生物群落的异质性结构尚不清楚。重要的是,地下微生物群落可能对异质环境中发现的任何数量的非生物、生物和空间驱动因素做出反应。在这里,我们研究了受威胁的佛罗里达灌木丛异质景观中土壤中原核和真菌群落的潜在驱动因素。佛罗里达灌木丛是一个热原生态系统,海拔高度的微小差异会导致水分、营养物质的可用性和植被组成的巨大变化。我们采用全面、大规模的采样设计来表征与三种生境类型和两种土壤深度(地壳和地下)相关的原核生物和真菌群落,以评估(i)这些异质生境中微生物群落的差异,(ii)非生物、生物和空间驱动因素在形成群落结构中的相对作用,以及(iii)真菌群落在这些栖息地的分布。我们对40个完整重复的栖息地×土壤深度组合的土壤进行了测序,并使用Illumina MiSeq对原核16S和真菌内部转录间隔区(ITS)区域进行了测序。生境异质性产生了不同的土壤原核生物和真菌群落。空间距离在构造地壳群落中发挥了作用,而地下微生物群落主要由灌木群落构成,它们可能与灌木群落的根相互作用。这一结果有助于解释我们在低海拔栖息地观察到的以丛枝菌根为主的土壤到高海拔栖息地以外生菌根为主的土地之间的意外转变。我们的研究结果挑战了以前微生物群落环境决定论的概念,并产生了关于异质环境共生关系的新假设。
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来源期刊
Mycologia
Mycologia 生物-真菌学
CiteScore
6.20
自引率
3.60%
发文量
56
审稿时长
4-8 weeks
期刊介绍: International in coverage, Mycologia presents recent advances in mycology, emphasizing all aspects of the biology of Fungi and fungus-like organisms, including Lichens, Oomycetes and Slime Molds. The Journal emphasizes subjects including applied biology, biochemistry, cell biology, development, ecology, evolution, genetics, genomics, molecular biology, morphology, new techniques, animal or plant pathology, phylogenetics, physiology, aspects of secondary metabolism, systematics, and ultrastructure. In addition to research articles, reviews and short notes, Mycologia also includes invited papers based on presentations from the Annual Conference of the Mycological Society of America, such as Karling Lectures or Presidential Addresses.
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