Biological function of C-X-C Motif Chemokine Ligand 1 gene (CXCL1) in ovarian malignant tumors.

Human & experimental toxicology Pub Date : 2023-01-01 DOI:10.1177/09603271231203392

Zhuang Li, Ning Huang, Wei Zhang, Li Li

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Abstract

Objective: To determine the function of the chemokine (C-X-C motif) ligand 1 (CXCL1) gene in ovarian cancer cells and to investigate the relationship between CXCL1 gene mRNA expression and ovarian tumor clinical pathology.

Methods: Using bioinformatics methods to identify common differentially expressed genes associated with ovarian cancer in the GEO database. Growth curves of A2780 cells with or without CXCL1 expression were plotted by MTT assay. Cell cycles were measured by flow cytometry. Cell colony formation was enumerated in Transwell chambers. Migration and invasion in vitro were investigated using Cell Counting Kit-8 (CCK8), wound healing and Transwell, respectively. The relationship between CXCL1 gene mRNA expression and ovarian tumor clinical pathology was analyzed.

Results: CXCL1 was found to be one of the co-upregulated differentially expressed genes in the GEO database. The migration of A2780 cells expressing CXCL1 was significantly higher than that of A2780 cells without CXCL1 expression. CXCL1 mRNA expression in ovarian malignancy was significantly higher than those in benign lesions and the normal control (p < .01). In advanced ovarian cancer (Stages III-IV), CXCL1 mRNA expression was also significantly higher than that in patients with early-stage ovarian cancer (Stages I-II) (p = .005). Kaplan-Meier survival curve showed no correlation between CXCL1 mRNA expression and ovarian cancer prognosis. A Cox proportional hazard model also showed that CXCL1 expression was not an independent prognostic factor for ovarian cancer patients.

Conclusions: CXCL1 gene could promotes ovarian cancer A2780 cell proliferation and invasion in vitro, and contributed theoretical knowledge for the target selection in molecular targeted therapy. CXCL1 mRNA over-expression may be correlated with the occurrence and development of ovarian malignancy. Level of plasma CXCL1 might serve as a biomarker for prognosis in ovarian carcinoma patients.

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C-X-C基序趋化因子配体1基因（CXCL1）在卵巢恶性肿瘤中的生物学功能。

目的：探讨趋化因子（C-X-C基序）配体1（CXCL1）基因在卵巢癌症细胞中的作用，探讨CXCL1基因mRNA表达与卵巢肿瘤临床病理的关系。方法：利用生物信息学方法，在GEO数据库中鉴定与卵巢癌症相关的常见差异表达基因。通过MTT法绘制具有或不具有CXCL1表达的A2780细胞的生长曲线。通过流式细胞术测量细胞周期。在Transwell室中计数细胞集落的形成。分别使用细胞计数试剂盒-8（CCK8）、伤口愈合和Transwell研究体外迁移和侵袭。分析CXCL1基因mRNA表达与卵巢肿瘤临床病理的关系。结果：CXCL1是GEO数据库中共同上调的差异表达基因之一。表达CXCL1的A2780细胞的迁移显著高于没有CXCL1表达的A2780。卵巢恶性肿瘤中CXCL1mRNA的表达显著高于良性病变和正常对照组（p＜0.01），CXCL1mRNA表达也显著高于早期卵巢癌症（I-II期）患者（p=.005）。Kaplan-Meier生存曲线显示CXCL1mmRNA表达与卵巢癌症预后无关。Cox比例风险模型还显示，CXCL1表达不是卵巢癌症患者的独立预后因素。结论：CXCL1基因可促进癌症A2780细胞的体外增殖和侵袭，为分子靶向治疗的靶点选择提供了理论依据。CXCL1mRNA过度表达可能与卵巢恶性肿瘤的发生发展有关。血浆CXCL1水平可能作为卵巢癌患者预后的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Human & experimental toxicology

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