Plasma activated water as a pre-treatment strategy in the context of biofilm-infected chronic wounds

IF 5.9 Q1 MICROBIOLOGY
Heema K.N. Vyas , Binbin Xia , David Alam , Nicholas P. Gracie , Joanna G. Rothwell , Scott A. Rice , Dee Carter , Patrick J. Cullen , Anne Mai-Prochnow
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Abstract

Healing and treatment of chronic wounds are often complicated due to biofilm formation by pathogens. Here, the efficacy of plasma activated water (PAW) as a pre-treatment strategy has been investigated prior to the application of topical antiseptics polyhexamethylene biguanide, povidone iodine, and MediHoney, which are routinely used to treat chronic wounds. The efficacy of this treatment strategy was determined against biofilms of Escherichia coli formed on a plastic substratum and on a human keratinocyte monolayer substratum used as an in vitro biofilm-skin epithelial cell model. PAW pre-treatment greatly increased the killing efficacy of all the three antiseptics to eradicate the E. coli biofilms formed on the plastic and keratinocyte substrates. However, the efficacy of the combined PAW-antiseptic treatment and single treatments using PAW or antiseptic alone was lower for biofilms formed in the in vitro biofilm-skin epithelial cell model compared to the plastic substratum. Scavenging assays demonstrated that reactive species present within the PAW were largely responsible for its anti-biofilm activity. PAW treatment resulted in significant intracellular reactive oxygen and nitrogen species accumulation within the E. coli biofilms, while also rapidly acting on the microbial membrane leading to outer membrane permeabilisation and depolarisation. Together, these factors contribute to significant cell death, potentiating the antibacterial effect of the assessed antiseptics.

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血浆活化水作为生物膜感染慢性伤口的预处理策略
由于病原体形成生物膜,慢性伤口的愈合和治疗往往很复杂。在这里,在应用常规用于治疗慢性伤口的局部防腐剂聚六亚甲基双胍、聚维酮碘和MediHoney之前,已经研究了血浆活化水(PAW)作为预处理策略的疗效。该处理策略对在塑料基质和用作体外生物膜皮肤上皮细胞模型的人角质形成细胞单层基质上形成的大肠杆菌生物膜的效力进行了测定。PAW预处理大大提高了所有三种防腐剂的杀灭效果,以根除在塑料和角质形成细胞基质上形成的大肠杆菌生物膜。然而,与塑料基质相比,在体外生物膜皮肤上皮细胞模型中形成的生物膜,联合PAW防腐处理和单独使用PAW或防腐处理的效果较低。清除试验表明,PAW中存在的活性物质在很大程度上负责其抗生物膜活性。PAW处理导致大肠杆菌生物膜内细胞内活性氧和氮物种的显著积累,同时也快速作用于微生物膜,导致外膜渗透和去极化。这些因素加在一起会导致显著的细胞死亡,增强所评估的防腐剂的抗菌效果。
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来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
期刊介绍:
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