Comparison of Corticosterone Concentrations in Dermal Secretions and Urine in Free-Ranging Marine Toads (Rhinella marina) in Human Care.

IF 1.9 Q2 VETERINARY SCIENCES

Veterinary Medicine International Pub Date : 2023-09-19 eCollection Date: 2023-01-01 DOI:10.1155/2023/1467549

Emma Clarke, Kimberly Ange-van Heugten, Troy N Tollefson, Frank N Ridgley, Dustin Smith, Janine L Brown, Heather Scott, Larry J Minter

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引用次数: 0

Abstract

Corticosterone concentrations have been measured in amphibians by collecting blood or urine samples. However, blood sampling is invasive, and urine can be difficult to collect. A novel method of swabbing the skin of an amphibian has been utilized in numerous species but has not been verified in marine toads (Rhinella marina). This pilot study tested dermal swabs as a noninvasive method for collecting and measuring dermal corticosterone secretions. Swabs were used to collect dermal secretion samples from sixty-six free-ranging marine toads collected on Zoo Miami grounds. The subsequent day the toads were shipped to the North Carolina Zoo where dermal samples were collected again. Additional dermal and urine samples were collected on days 9, 15, 32, and 62 under human care to measure corticosterone concentrations. There was no significant correlation (P ≥ 0.05) noted between corticosterone concentrations reported in dermal swabs and those in urine samples at all four of the euthanasia time points or between the corticosterone concentrations reported in either urine or dermal swabs and the weight of the toads. Dermal swab concentrations (ng/mL) were significantly higher (P ≤ 0.05) on the day of capture (0.64 ± 0.03) and the day of arrival (0.67 ± 0.03) than on day 15 (0.47 ± 0.03). The urine corticosterone concentrations decreased while the toads were in human care with a significant decrease (P ≤ 0.05) between days 9 (0.45 ± 0.07) and 32 (0.21 ± 0.06). This study demonstrated that dermal swabs can be used to collect marine toad corticosterone concentration samples.

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人类护理中自由放养海洋蟾蜍（Rhinella marina）皮肤分泌物和尿液中皮质酮浓度的比较。

通过采集血液或尿液样本来测量两栖动物的皮质酮浓度。然而，血液采样是侵入性的，尿液可能很难收集。一种新的擦拭两栖动物皮肤的方法已经在许多物种中使用，但尚未在海洋蟾蜍（Rhinella marina）中得到验证。这项初步研究测试了皮肤拭子作为一种非侵入性方法来收集和测量皮肤皮质酮分泌物。用拭子从迈阿密动物园采集的66只自由放养的海洋蟾蜍身上采集皮肤分泌物样本。第二天，蟾蜍被运往北卡罗来纳州动物园，在那里再次采集了皮肤样本。在人类护理下于第9、15、32和62天采集额外的皮肤和尿液样本，以测量皮质酮浓度。无显著相关性（P ≥ 0.05）在所有四个安乐死时间点的皮拭子中报告的皮质酮浓度与尿液样本中的皮质酮含量之间，或者在尿液或皮拭子报告的皮质类固醇浓度与蟾蜍的重量之间。皮肤拭子浓度（ng/mL）显著高于对照组（P ≤ 0.05）（0.64 ± 0.03）和到达日期（0.67 ± 0.03）比第15天（0.47 ± 0.03）。蟾蜍在人类护理期间尿皮质酮浓度下降，但有显著下降（P ≤ 0.05）（0.45 ± 0.07）和32（0.21 ± 0.06）。该研究表明，真皮拭子可用于采集海洋蟾蜍皮质酮浓度样本。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Medicine International Veterinary-Veterinary (all)

CiteScore

3.50

自引率

3.20%

发文量

审稿时长

17 weeks

期刊介绍： Veterinary Medicine International is a peer-reviewed, Open Access journal that publishes original research articles and review articles in all areas of veterinary research. The journal will consider articles on the biological basis of disease, as well as diagnosis, prevention, treatment, and epidemiology.