Absorption of egg white hydrolysate in the intestine: Clathrin-dependent endocytosis as the main transport route.

Haixi You, Juanrui Li, Yiju Li, Wei Wang, Zhipeng Yu, Jingbo Liu, Xuebo Liu, Long Ding
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Abstract

This paper aimed to investigate the in vivo absorption of egg white hydrolysate (EWH) in rats and the transport route across the intestinal epithelium. Results showed that the level of plasma peptide-bound amino acid (PAA) of the EWH-supplemented rats (EWH-R) was determined to be 2012.18 ± 300.98 μmol/L, 10.72% higher than that of the control group, and was significantly positively correlated to that of EWH. Thirty-three egg white-derived peptides were successfully identified from the plasma of EWH-R, and 20 of them were found in both EWH-R plasma and EWH, indicating that these peptides tend to be absorbed through the intestinal epithelium in intact forms into the blood circulation. In addition, 637 up-regulated and 577 down-regulated genes in Caco-2 cells incubated with EWH were detected by RNA-sequencing and the clathrin-dependent endocytosis was the most enriched pathway in KEGG analysis. EWH significantly increased the mRNA levels of the key genes involved in the clathrin-dependent endocytosis but these changes would be inhibited by the clathrin-dependent endocytosis inhibitor of chlorpromazine. Moreover, the transepithelial transport of EWH across Caco-2 cell monolayers was significantly reduced by chlorpromazine. This study provided molecular-level evidence for the first time that clathrin-dependent endocytosis might be the main transport route of EWH in the intestinal epithelium.

蛋清水解产物在肠道中的吸收:氯氰菊酯依赖的内吞作用是主要的转运途径。
本文旨在研究蛋清水解物(EWH)在大鼠体内的吸收以及通过肠上皮的转运途径。结果显示,EWH补充组大鼠血浆肽结合氨基酸(PAA)水平为2012.18±300.98μmol/L,比对照组高10.72%,与EWH水平呈正相关。从EWH-R的血浆中成功鉴定出33种蛋白衍生肽,其中20种在EWH-R血浆和EWH中都发现,这表明这些肽倾向于以完整的形式通过肠上皮吸收到血液循环中。此外,通过RNA测序,在与EWH孵育的Caco-2细胞中检测到637个上调和577个下调基因,网格蛋白依赖性内吞作用是KEGG分析中最丰富的途径。EWH显著增加了参与网格蛋白依赖性内吞作用的关键基因的mRNA水平,但这些变化将被氯丙嗪的网格蛋白依赖型内吞作用抑制剂抑制。此外,氯丙嗪显著降低了EWH通过Caco-2细胞单层的跨上皮转运。这项研究首次提供了分子水平的证据,证明网格蛋白依赖的内吞作用可能是EWH在肠上皮中的主要转运途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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