High rumen degradable starch diet induced blood bile acids profile changes and hepatic inflammatory response in dairy goats

IF 6.3
Lixin Zheng , Jing Shen , Xiaoying Han , Chunjia Jin , Xiaodong Chen , Junhu Yao
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引用次数: 0

Abstract

The objective of this study was to reveal the effect of rumen degradable starch (RDS) on bile acid metabolism and liver transcription in dairy goats using metabolomics and transcriptomics. Eighteen Guanzhong dairy goats of a similar weight and production level (body weight = 45.8 ± 1.54 kg, milk yield = 1.75 ± 0.08 kg, and second parity) were randomly assigned to 3 treatment groups where they were fed a low RDS (LRDS, RDS = 20.52% DM) diet, medium RDS (MRDS, RDS = 22.15% DM) diet, or high RDS (HRDS, RDS = 24.88% DM) diet, respectively. The goats were fed with the experimental diets for 5 weeks. On the last day of the experiment, all goats were anesthetized, and peripheral blood and liver tissue samples were collected. The peripheral blood samples were used in metabolomic analysis and white blood cell (WBC) count, whereas the liver tissue samples were used in transcriptomic analysis. Based on the metabolomics results, the relative abundances of primary bile acids in the peripheral blood were significantly reduced in the group that was fed the HRDS diet (P < 0.05). The WBC count was significantly increased in the HRDS group compared with that in the LRDS and MRDS groups (P < 0.01), indicating that there was inflammation in the HRDS group. Transcriptomic analysis showed that 4 genes related to bile acid secretion (genes: MDR1, RXRα, AE2, SULT2A1) were significantly downregulated in the HRDS group. In addition, genes related to the immune response were upregulated in the HRDS group, suggesting the HRDS diet induced a hepatic inflammatory response mediated by lipopolysaccharides (LPS) (gene: LBP), activated the Toll-like receptor 4 binding (genes: S100A8, S100A9) and the NF-kappa B signaling pathway (genes: LOC106503980, LOC108638497, CD40, LOC102180880, LOC102170970, LOC102175177, LBP, LOC102168903, LOC102185461, LY96 and CXCL8), triggered inflammation and complement responses (genes: C1QB, C1QC, and CFD). The HRDS diet induced a hepatic inflammatory response may be mediated by activating the Toll-like receptor 4 binding and NF-kappa B signaling pathway after free LPS entered the liver. The changes of bile acids profile in blood and the down-regulation of 4 key genes (MDR1, RXRα, AE2, SULT2A1) involved in bile secretion in liver are probably related to liver inflammation.

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高瘤胃可降解淀粉日粮诱导奶山羊血液胆汁酸谱变化和肝脏炎症反应。
本研究的目的是利用代谢组学和转录组学揭示瘤胃可降解淀粉(RDS)对奶山羊胆汁酸代谢和肝脏转录的影响。将18只体重和生产水平相似(体重=45.8±1.54kg,产奶量=1.75±0.08kg,二产)的关中奶山羊随机分为3个处理组,分别饲喂低RDS(LRDS,RDS=20.52%DM)日粮、中RDS(MRDS,RDD=22.15%DM)日食或高RDS(HRDS,RDS=24.88%DM)日餐。用实验日粮喂养山羊5周。在实验的最后一天,将所有山羊麻醉,并采集外周血和肝组织样本。外周血样本用于代谢组学分析和白细胞计数,而肝组织样本用于转录组学分析。根据代谢组学结果,在喂食HRDS饮食的组中,外周血中初级胆汁酸的相对丰度显著降低(P MDR1、RXRα、AE2、SULT2A1)在HRDS组中显著下调。此外,与免疫反应相关的基因在HRDS组中上调,表明HRDS饮食诱导了由脂多糖(LPS)介导的肝脏炎症反应(基因:LBP),激活Toll样受体4结合(基因:S100A8、S100A9)和NF-κB信号通路(基因:LOC106503980、LOC108638497、CD40、LOC102180880、LOC102170970、LOC102175177、LBP、LOC102168903、LOC102185461、LY96和CXCL8),触发炎症和补体反应(基因:C1QB、C1QC和CFD)。HRDS饮食诱导的肝脏炎症反应可以通过在游离LPS进入肝脏后激活Toll样受体4结合和NF-κB信号通路来介导。血液中胆汁酸谱的变化和参与肝脏胆汁分泌的4个关键基因(MDR1、RXRα、AE2、SULT2A1)的下调可能与肝脏炎症有关。
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来源期刊
Animal Nutrition
Animal Nutrition Animal Science and Zoology
CiteScore
9.70
自引率
0.00%
发文量
542
审稿时长
65 days
期刊介绍: Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to primarily to the nutrition of farm animals and aquatic species. More applied aspects of animal nutrition, such as the evaluation of novel ingredients, feed additives and feed safety will also be considered but it is expected that such studies will have a strong nutritional focus. Animal Nutrition is indexed in SCIE, PubMed Central, Scopus, DOAJ, etc.
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