Comparative analysis of repetitive DNA in dysploid and non-dysploid Phaseolus beans.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Maria Eduarda Ferraz, Tiago Ribeiro, Mariela Sader, Thiago Nascimento, Andrea Pedrosa-Harand
{"title":"Comparative analysis of repetitive DNA in dysploid and non-dysploid Phaseolus beans.","authors":"Maria Eduarda Ferraz, Tiago Ribeiro, Mariela Sader, Thiago Nascimento, Andrea Pedrosa-Harand","doi":"10.1007/s10577-023-09739-3","DOIUrl":null,"url":null,"abstract":"<p><p>Structural karyotype changes result from ectopic recombination events frequently associated with repetitive DNA. Although most Phaseolus species present relatively stable karyotypes with 2n = 22 chromosomes, the karyotypes of species of the Leptostachyus group show high rates of structural rearrangements, including a nested chromosome fusion that led to the dysploid chromosome number of the group (2n = 20). We examined the roles of repetitive landscapes in the rearrangements of species of the Leptostachyus group using genome-skimming data to characterize the repeatome in a range of Phaseolus species and compared them to species of that group (P. leptostachyus and P. macvaughii). LTR retrotransposons, especially the Ty3/gypsy lineage Chromovirus, were the most abundant elements in the genomes. Differences in the abundance of Tekay, Retand, and SIRE elements between P. macvaughii and P. leptostachyus were reflected in their total amounts of Ty3/gypsy and Ty1/copia. The satellite DNA fraction was the most divergent among the species, varying both in abundance and distribution, even between P. leptostachyus and P. macvaughii. The rapid turnover of repeats in the Leptostachyus group may be associated with the several rearrangements observed.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10577-023-09739-3","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0

Abstract

Structural karyotype changes result from ectopic recombination events frequently associated with repetitive DNA. Although most Phaseolus species present relatively stable karyotypes with 2n = 22 chromosomes, the karyotypes of species of the Leptostachyus group show high rates of structural rearrangements, including a nested chromosome fusion that led to the dysploid chromosome number of the group (2n = 20). We examined the roles of repetitive landscapes in the rearrangements of species of the Leptostachyus group using genome-skimming data to characterize the repeatome in a range of Phaseolus species and compared them to species of that group (P. leptostachyus and P. macvaughii). LTR retrotransposons, especially the Ty3/gypsy lineage Chromovirus, were the most abundant elements in the genomes. Differences in the abundance of Tekay, Retand, and SIRE elements between P. macvaughii and P. leptostachyus were reflected in their total amounts of Ty3/gypsy and Ty1/copia. The satellite DNA fraction was the most divergent among the species, varying both in abundance and distribution, even between P. leptostachyus and P. macvaughii. The rapid turnover of repeats in the Leptostachyus group may be associated with the several rearrangements observed.

Abstract Image

异倍体和非异倍体菜豆重复DNA的比较分析。
结构核型变化是由经常与重复DNA相关的异位重组事件引起的。尽管大多数Phaseolus物种呈现出相对稳定的2n核型 = 22条染色体,瘦端壶菌群物种的核型显示出高的结构重排率,包括嵌套的染色体融合,导致该群的染色体数目异常(2n = 20) 。我们使用基因组浏览数据来表征一系列Phaseolus物种中的重复体,并将其与该组物种(P.Leptostachyus和P.macvaughii)进行比较,研究了重复景观在Leptostachius组物种重排中的作用。LTR逆转录转座子,特别是Ty3/吉普赛谱系色病毒,是基因组中最丰富的元素。macvaughii和Leptostachhyus之间Tekay、Retand和SIRE元素丰度的差异反映在它们的Ty3/gypsy和Ty1/copia总量中。卫星DNA部分在各物种中差异最大,在丰度和分布上都有所不同,甚至在钩端藻和麦氏藻之间也是如此。瘦素组重复序列的快速周转可能与观察到的几种重排有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信