CCA repair or ECA ligation—Which middle cerebral artery occlusion is better in the reperfusion mouse model?

Ibrain Pub Date : 2023-08-18 DOI:10.1002/ibra.12128
Yue Hu, Zhen-Hong Yang, Feng Yan, Shuang-Feng Huang, Rong-Liang Wang, Zi-Ping Han, Jun-Fen Fan, Yang-Min Zheng, Ping Liu, Yu-Min Luo, Si-Jie Li
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Abstract

A reliable animal model is essential for ischemic stroke research. The implications of the external carotid artery (ECA) transection or common carotid artery (CCA) ligation have been described. Thus, a modified animal model, the CCA-repair model, has been established, and studies have shown that the CCA-repair model has potential advantages over the CCA-ligation model. However, whether the CCA-repair model is superior to the ECA-ligation model remains unclear. Sixty male C57BL/6 mice were randomly assigned to establish the CCA-repair (n = 34) or ECA-ligation (n = 26) models. Cerebral blood flow before middle cerebral artery occlusion (MCAO), immediately after MCAO and reperfusion were monitored and the operation duration, postoperative body weight, and food intake within 7 days, and the number of intraoperative and postoperative deaths within 7 days were recorded in the two models. Modified neurological severity scores and Bederson (0–5) scores were used to evaluate postoperative neurological function deficits on Days 1/3/5/7. 2,3,5-Triphenyltetrazolium chloride staining was used to quantify lesion volume on Day 7 after the operation. We found the establishment of the CCA-repair model required a longer total operation duration (p = 0.0175), especially the operation duration of reperfusion (p < 0.0001). However, there was no significant difference in body weight and food intake development, lesion volume and intragroup variability, neurological function deficits, mortality, and survival probability between the two groups. The CCA-repair model has no significant advantage over the ECA-ligation model. The ECA-ligation model is still a better choice for focal cerebral ischemia.

Abstract Image

CCA修复还是ECA结扎在再灌注小鼠模型中哪种大脑中动脉闭塞更好?
一个可靠的动物模型对缺血性中风的研究至关重要。已经描述了颈外动脉(ECA)横断或颈总动脉(CCA)结扎的含义。因此,已经建立了一种改良的动物模型,即CCA修复模型,并且研究表明CCA修复模式比CCA结扎模式具有潜在的优势。然而,CCA修复模型是否优于ECA结扎模型尚不清楚。60只雄性C57BL/6小鼠被随机分配以建立CCA修复(n = 34)或ECA结扎(n = 26)模型。监测大脑中动脉闭塞(MCAO)前、MCAO和再灌注后即刻的脑血流量,并记录两个模型的手术时间、术后体重、7天内的食物摄入量以及7天内术中和术后死亡人数。在第1/3/5/7天,使用改良的神经严重程度评分和Bederson(0-5)评分来评估术后神经功能缺损。2,3,5-三苯基氯化四氮唑染色用于量化手术后第7天的病变体积。我们发现CCA修复模型的建立需要更长的总操作持续时间(p = 0.0175),尤其是再灌注的手术时间(p
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