miRNA-15a, miRNA-15b, and miRNA-499 are Reduced in Erythrocytes of Pre-Diabetic African-American Adults.

Jacobs journal of diabetes and endocrinology Pub Date : 2016-12-01 Epub Date: 2016-11-15
Maurice B Fluitt, Namita Kumari, Gail Nunlee-Bland, Sergei Nekhai, Kanwal K Gambhir
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Abstract

Aims: The use of circulatory miRNAs as biomarkers and therapeutic targets for T2DM is an explosive area of study. However, no study has investigated circulatory miRNA expression exclusively in African-American adults. The aim of this study was to identify the expression of nine selected miRNAs in erythrocytes of pre-diabetic and type 2 diabetic African-American adults.

Main methods: Patients were recruited from the Howard University Hospital Diabetes Treatment Center following an 8 to 10 hour overnight fast. Expression of the nine selected miRNAs (miRNA-499, miRNA-146, miRNA-126, miRNA-223, miRNA-15a, miRNA-15b, miRNA-224, miRNA-326, and miRNA-375) was evaluated using quantitative real time PCR.

Key findings: miRNA-15a, miRNA-15b, and miRNA-499 were significantly reduced in erythrocytes of pre-diabetic African-American adults. In the T2DM group, we found significant correlations between miRNA-15a and BMI (r=0.59, p=0.04), miRNA-15a and weight (r=0.52, p=0.01), and miRNA-15b and diastolic blood pressure (r=-0.52, p=0.02). In the pre-diabetic group, we found significant correlations between miRNA-15b and weight (r=0.90, p=0.02) and miRNA-499 and HbA1c (r=-0.89, p=0.01).

Significance: To our knowledge, this is the first study investigating miRNA expression in erythrocytes of non-diabetic high-risk obese--pre-diabetic and type 2 diabetic African-American adults. The findings of this study are consistent with previous reports of reduced expression of miRNA-15a, miRNA-15b, and miRNA-499 in human plasma or serum and in animal models. The current findings support the use of circulating miRNA-15a, miRNA-15b, and miRNA-499 as potential biomarkers for T2DM in African-American adults.

Abstract Image

Abstract Image

miRNA-15a、miRNA-15b和miRNA-499在糖尿病前期非裔美国成年人的红细胞中减少。
目的:使用循环miRNA作为T2DM的生物标志物和治疗靶点是一个爆炸性的研究领域。然而,没有研究专门研究循环miRNA在非裔美国成年人中的表达。本研究的目的是鉴定9种选定的miRNA在糖尿病前期和2型糖尿病非裔美国成年人红细胞中的表达。主要方法:患者在霍华德大学医院糖尿病治疗中心接受8至10小时的通宵禁食。使用实时定量PCR评估九种选定miRNA(miRNA-499、miRNA-146、miRNA-126、miRNA-223、miRNA-15a、miRNA15b、miRNA-224、miRNA-326和miRNA-375)的表达。关键发现:糖尿病前期非裔美国成年人的红细胞中miRNA-15b、miRNA-15a显著减少。在T2DM组中,我们发现miRNA-15a与BMI(r=0.59,p=0.04)、miRNA-15a与体重(r=0.52,p=0.01)、miRNA-15b与舒张压(r=-0.52,p=0.02)之间存在显著相关性,这是第一项研究miRNA在非糖尿病高危肥胖——糖尿病前期和2型糖尿病非裔美国成年人红细胞中的表达。这项研究的发现与之前关于miRNA-15a、miRNA-15b和miRNA-499在人类血浆或血清以及动物模型中表达减少的报道一致。目前的研究结果支持使用循环miRNA-15a、miRNA-15b和miRNA-499作为非裔美国成年人T2DM的潜在生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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