H19 Promoter DNA Methylation is Lower Among Early Abortion Patients Undergoing IVF Embryo Transfer.

IF 1.2 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Lei Han, Xianghui Zhang, Xiaohan Wu, Huishu Xu, Baolin Zhang, Yiwei Pang, Peihui Ding, Caiying Zhang, Yanlin Wang, Jiajia An
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引用次数: 0

Abstract

Background: H19 is the first long noncoding RNA (lncRNA) found to be associated with gene imprinting. It is highly expressed in the embryonic stage and may have important regulatory effects on human embryonic development. We investigated the differences between the levels of H19 promoter DNA methylation in the chorionic villi of patients who experienced spontaneous abortion (SA) following in vitro fertilization embryo transfer (IVF-ET) and those of patients with a normal early pregnancy (NEP). We also analyzed the associated DNA methyltransferase (DNMT) activity.

Methods: Chorionic villus tissue from patients with SA and NEP were collected. The DNA methylation levels of two CpG islands in the promoter region of the H19 gene in the two groups were detected by bisulfite sequencing, and the mRNA expression of DNMTs was analyzed by real-time polymerase chain reaction.

Results: The sample size of each group was 32, and there were no significant differences in baseline data, including age, parity, and body mass index, between the two groups. Among the 7 CpG islands measured, the methylation rates of 3 CpG islands (CpG 1, 6, and 7) were significantly lower in the SA group than in the NEP group (P < 0.01). The methylation levels of the other 4 CpG islands were not significantly different between the two groups. There were no differences in the expression of DNMT1 between the two groups (P > 0.05), but DNMT3a and DNMT3b RNA levels were significantly lower in SA group than in the NEP group (P < 0.01).

Conclusions: The lower H19 promoter DNA methylation levels found in the chorionic villi of patients with SA patients following IVF-ET may be explained by decreased expression of DNMT3a and DNMT3b.

H19启动子DNA甲基化在接受试管婴儿胚胎移植的早期流产患者中较低。
背景:H19是第一个被发现与基因印迹相关的长非编码RNA(lncRNA)。它在胚胎阶段高度表达,可能对人类胚胎发育具有重要的调节作用。我们研究了体外受精-胚胎移植(IVF-ET)后自然流产(SA)患者和正常早孕(NEP)患者绒毛膜中H19启动子DNA甲基化水平的差异。我们还分析了相关的DNA甲基转移酶(DNMT)活性。方法:收集SA和NEP患者的绒毛组织。通过亚硫酸氢盐测序检测两组H19基因启动子区两个CpG岛的DNA甲基化水平,并通过实时聚合酶链反应分析DNMTs的mRNA表达。结果:每组的样本量为32,两组之间的基线数据(包括年龄、产次和体重指数)没有显著差异。在7个CpG岛中,SA组的3个CpG岛屿(CpG1、6和7)的甲基化率显著低于NEP组(P<0.01)。其他4个CpG群岛的甲基化水平在两组之间没有显著差异。两组间DNMT1表达无差异(P>0.05),但SA组DNMT3a和DNMT3b RNA水平明显低于NEP组(P<0.01)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Clinical and Investigative Medicine
Clinical and Investigative Medicine 医学-医学:研究与实验
CiteScore
1.50
自引率
12.50%
发文量
18
审稿时长
>12 weeks
期刊介绍: Clinical and Investigative Medicine (CIM), publishes original work in the field of Clinical Investigation. Original work includes clinical or laboratory investigations and clinical reports. Reviews include information for Continuing Medical Education (CME), narrative review articles, systematic reviews, and meta-analyses.
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