Protective effect of Lycium barbarum leaf extracts on atopic dermatitis: in vitro and in vivo studies.

IF 2 4区 医学 Q3 NUTRITION & DIETETICS
Nutrition Research and Practice Pub Date : 2023-10-01 Epub Date: 2023-07-11 DOI:10.4162/nrp.2023.17.5.855
Han Sol Lee, Eun Young Bae, Sun Yung Ly
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引用次数: 0

Abstract

Background/objectives: Atopic dermatitis (AD) is a chronic disease with an increasing incidence globally; therefore, there is a growing demand for natural compounds effective in treating dermatitis. In this study, the protective effects of Lycium barbarum leaves with and without chlorophyll (LLE and LLE[Ch-]) on AD were investigated in animal models of AD and HaCaT cells. Further, we investigated whether LLE and LLE(Ch-) show any differences in physiological activity.

Materials/methods: AD was induced by 2,4-dinitrochlorobenzene (DNCB) for three weeks, while NC/Nga mice were fed LLE or LLE(Ch-) extracts for 7 weeks. Serum immunoglobulin E (IgE) and cytokine (tumor necrosis factor [TNF]-α, interleukin [IL]-6, and IL-4) concentrations and the degree of DNA fragmentation in lymphocytes were examined. A histopathological examination (haematoxylin & eosin staining and blue spots of toluidine) of the dorsal skin of mice was performed. To elucidate the mechanism of action, the expression of the thymus and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC) were measured in HaCaT cells.

Results: Serum IgE and cytokines (TNF-α and IL-6) levels as well as DNA fragmentation of lymphocytes were significantly decreased in AD-induced mice treated with LLE or LLE(Ch-) compared to those of the control group. The epidermal thickness of the dorsal skin and mast cell infiltration in the LLE group significantly reduced compared to that in the control group. The LLE extracts showed no cytotoxicity up to 1,000 µg/mL in HaCaT cells. LLE or LLE(Ch-)-treated group showed a reduction of TARC and MDC in TNF-α-and IFN-γ-stimulated HaCaT cells.

Conclusions: These results suggest that LLE potentially improves inflammation by reducing the expression of chemokines that inhibit T helper 2 cell migration. LLE(Ch-) showed similar effects to LLE on blood levels of IgE, TNF-α and IL-6 and protein expression in HaCat cells, but the ultimate effect of skin improvement was not statistically significant. Therefore, both LLE and LLE(Ch-) can be used as functional materials to alleviate AD, but LLE(Ch-) appears to require more research to improve inflammation.

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枸杞叶提取物对特应性皮炎的保护作用:体外和体内研究。
背景/目的:特应性皮炎(AD)是一种在全球发病率不断上升的慢性疾病;因此,对治疗皮炎有效的天然化合物的需求越来越大。在本研究中,在AD和HaCaT细胞的动物模型中研究了含有和不含有叶绿素(LLE和LLE[Ch-])的枸杞叶对AD的保护作用。此外,我们还研究了LLE和LLE(Ch-)在生理活性方面是否存在任何差异。材料/方法:2,4-二硝基氯苯(DNCB)诱导AD 3周,同时给予NC/Nga小鼠LLE或LLE(Ch-)提取物7周。检测血清免疫球蛋白E(IgE)和细胞因子(肿瘤坏死因子[TNF]-α、白细胞介素[IL]-6和IL-4)的浓度以及淋巴细胞中DNA断裂的程度。对小鼠背部皮肤进行组织病理学检查(苏木精和伊红染色和甲苯胺蓝斑)。为了阐明其作用机制,测定了HaCaT细胞中胸腺和活化调节趋化因子(TARC)以及巨噬细胞衍生趋化因子的表达。结果:与对照组相比,LLE或LLE(Ch-)治疗的AD诱导小鼠血清IgE和细胞因子(TNF-α和IL-6)水平以及淋巴细胞DNA断裂显著降低。LLE组的背侧皮肤表皮厚度和肥大细胞浸润与对照组相比显著减少。LLE提取物在HaCaT细胞中显示出高达1000µg/mL的细胞毒性。LLE或LLE(Ch-)处理组显示TNF-α和IFN-γ刺激的HaCaT细胞的TARC和MDC降低。结论:这些结果表明,LLE可能通过减少抑制T辅助细胞2迁移的趋化因子的表达来改善炎症。LLE(Ch-)对血液中IgE、TNF-α和IL-6水平以及HaCat细胞中蛋白质表达的影响与LLE相似,但皮肤改善的最终效果没有统计学意义。因此,LLE和LLE(Ch-)都可以作为减轻AD的功能材料,但LLE(Ch-)似乎需要更多的研究来改善炎症。
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来源期刊
Nutrition Research and Practice
Nutrition Research and Practice NUTRITION & DIETETICS-
CiteScore
3.50
自引率
4.20%
发文量
62
审稿时长
6-12 weeks
期刊介绍: Nutrition Research and Practice (NRP) is an official journal, jointly published by the Korean Nutrition Society and the Korean Society of Community Nutrition since 2007. The journal had been published quarterly at the initial stage and has been published bimonthly since 2010. NRP aims to stimulate research and practice across diverse areas of human nutrition. The Journal publishes peer-reviewed original manuscripts on nutrition biochemistry and metabolism, community nutrition, nutrition and disease management, nutritional epidemiology, nutrition education, foodservice management in the following categories: Original Research Articles, Notes, Communications, and Reviews. Reviews will be received by the invitation of the editors only. Statements made and opinions expressed in the manuscripts published in this Journal represent the views of authors and do not necessarily reflect the opinion of the Societies.
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