Antibiotic resistance and virulence potentials of E. faecalis and E. faecium in hospital wastewater: a case study in Ardabil, Iran.

IF 2.5 4区环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES

Journal of water and health Pub Date : 2023-09-01 DOI:10.2166/wh.2023.147

Elham Jannati, Farzad Khademi, Meysam Manouchehrifar, Dadras Maleki, Nour Amirmozaffari, Vajihe Sadat Nikbin, Mohsen Arzanlou

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Abstract

Hospital wastewater can contaminate the environment with antibiotic-resistant and virulent bacteria. We analyzed wastewater samples from four hospitals in Ardabil province, Iran for Enterococcus faecium and Enterococcus faecalis using culture and molecular methods. We also performed antimicrobial susceptibility testing and polymerase chain reaction testing for resistance and virulence genes. Out of 141 enterococci isolates, 68.8% were E. faecium and 23.4% were E. faecalis. Ciprofloxacin and rifampicin showed the highest level of resistance against E. faecalis and E. faecium isolates at 65%. High-level gentamicin resistance (HLGR), high-level streptomycin resistance (HLSR), ampicillin, and vancomycin resistance were observed in 25, 5, 10, and 5.15% of E. faecium, and 15, 6, 15, and 3.03% of E. faecalis isolates, respectively. The ant(6')-Ia and ant(3')-Ia genes that were responsible for streptomycin resistance were observed in HLSR isolates and aph(3')-IIIa and aac(6') Ie-aph(2″)-Ia genes accounting for gentamicin resistance were detected in HLGR isolates. vanA was the predominant gene detected in vancomycin-resistant isolates. The majority of isolates were positive for gelE, asa1, esp, cylA, and hyl virulence genes. We found that drug-resistant and virulent E. faecalis and E. faecium isolates were prevalent in hospital wastewater. Proper treatment strategies are required to prevent their dissemination into the environment.

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医院废水中粪大肠杆菌和粪大肠杆菌的抗生素耐药性和毒力潜力：伊朗Ardabil的一项案例研究。

医院废水可能会污染环境中的抗生素耐药性和毒性细菌。我们使用培养和分子方法分析了伊朗阿达比尔省四家医院的废水样本中的粪肠球菌和粪肠球菌。我们还对耐药性和毒力基因进行了抗菌药物敏感性测试和聚合酶链式反应测试。141株肠球菌中，68.8%为粪肠球菌，23.4%为粪肠球菌。环丙沙星和利福平对粪大肠杆菌和粪大肠杆菌分离株的耐药性最高，为65%。在25、5、10和5.15%的粪肠球菌和15、6、15和3.03%的粪肠球菌分离株中分别观察到高水平庆大霉素耐药性（HLGR）、高水平链霉素耐药性（HLSR）、氨苄青霉素和万古霉素耐药性。在HLSR分离株中观察到与链霉素耐药性有关的ant（6’）-Ia和ant（3’）-Ia基因，在HLGR分离株中检测到与庆大霉素耐药性有关的aph（3’’）-IIIa和aac（6’’）Ie-aph（2〃）-Ia基因。vanA是在万古霉素耐药菌株中检测到的主要基因。大多数分离株对gelE、asa1、esp、cylA和hyl毒力基因呈阳性。我们发现，医院废水中普遍存在抗药性和毒力的粪大肠杆菌和粪大肠杆菌分离株。需要采取适当的治疗策略，防止其传播到环境中。

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来源期刊

Journal of water and health 环境科学-环境科学

CiteScore

3.60

自引率

8.70%

发文量

110

审稿时长

18-36 weeks

期刊介绍： Journal of Water and Health is a peer-reviewed journal devoted to the dissemination of information on the health implications and control of waterborne microorganisms and chemical substances in the broadest sense for developing and developed countries worldwide. This is to include microbial toxins, chemical quality and the aesthetic qualities of water.