Inter-laboratory validation of liquid chromatography-tandem mass spectrometry multi-mycotoxin determination in animal feed - method transfer from the reference laboratory to regional laboratories.

IF 1.3 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Veterinary Research Pub Date : 2023-09-20 eCollection Date: 2023-09-01 DOI:10.2478/jvetres-2023-0045

Piotr Jedziniak, Łukasz Panasiuk, Katarzyna Pietruszka, Krzysztof Kolano, Radosław Zbytniewski

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Abstract

Introduction: The results are presented of the inter-laboratory validation of a liquid chromatography-tandem mass spectrometry method for the determination of eight mycotoxins (aflatoxin B1, deoxynivalenol, fumonisin B1, fumonisin B2, ochratoxin A, toxin T-2, toxin HT-2 and zearalenone) in animal feeds.

Material and methods: This study was an essential part of the method's transfer from the National Reference Laboratory to six regional laboratories in Poland working in the official survey of mycotoxins in feed. The laboratories received a batch of standard solutions, blank samples and quality control materials on which to perform analysis with one procedure and different liquid chromatography-tandem mass spectrometry conditions.

Results: The validation results show good precision (reproducibility coefficient of variation 3.7-20.5%) and accuracy of the method (recovery 89-120% and trueness 94-103%) and sufficient skills of the laboratory personnel.

Conclusion: The study is an example of the successful transfer of the method among laboratories.

Abstract Image

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液相色谱-串联质谱法测定动物饲料中多种真菌毒素的实验室间验证——方法从参考实验室转移到区域实验室。

前言：本文介绍了液相色谱-串联质谱法测定动物饲料中8种真菌毒素（黄曲霉毒素B1、脱氧雪腐镰刀菌烯醇、伏马菌素B1、伏马毒素B2、赭曲霉毒素a、毒素T-2、毒素HT-2和玉米赤霉烯酮）的实验室间验证结果。材料和方法：这项研究是该方法从国家参考实验室转移到波兰六个地区实验室的重要组成部分，这些实验室致力于饲料中真菌毒素的官方调查。实验室收到了一批标准溶液、空白样品和质量控制材料，用一种程序和不同的液相色谱-串联质谱条件对其进行分析。结果：验证结果表明，该方法具有良好的精密度（重现性变异系数3.7-20.5%）和准确度（回收率89-120%，准确度94-103%），实验室人员有足够的技能。结论：该研究是该方法在实验室间成功转移的一个例子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Research Veterinary-General Veterinary

CiteScore

0.90

自引率

5.60%

发文量

审稿时长

18 weeks

期刊介绍： Journal of Veterinary Research (formerly Bulletin of the Veterinary Institute in Pulawy) is a quarterly that publishes original papers, review articles and short communications on bacteriology, virology, parasitology, immunology, molecular biology, pathology, toxicology, pharmacology, and biochemistry. The main emphasis is, however, on infectious diseases of animals, food safety and public health, and clinical sciences.