ER body-resident myrosinases and tryptophan specialized metabolism modulate root microbiota assembly

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2023-09-28 DOI:10.1111/nph.19289

Arpan Kumar Basak, Anna Piasecka, Jana Hucklenbroich, Gözde Merve Türksoy, Rui Guan, Pengfan Zhang, Felix Getzke, Ruben Garrido-Oter, Stephane Hacquard, Kazimierz Strzałka, Paweł Bednarek, Kenji Yamada, Ryohei Thomas Nakano

引用次数: 0

Abstract

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ER体内固有的肌苷酶和色氨酸专门代谢调节根微生物群的组装。

内质网（ER）体是内质网衍生的结构，含有大量PYK10月桂糖苷酶，可水解色氨酸（Trp）衍生的吲哚硫代葡萄糖苷（IGs）。鉴于IGs在根-微生物相互作用中的作用已被充分描述，我们假设根中的ER体对于在根-土界面与土传微生物的相互作用很重要。我们使用ER体受损的突变体（nai1）、ER体驻留的myrosinase（pyk10bglu21）、IG生物合成（myb34/51/122）和Trp专门代谢（cyp79b2b3）来分析其在天然土壤中的根系微生物群群落，评估轴向收集的根系分泌物对土壤或合成微生物群落的影响，并在单关联设置中测试它们对真菌内生菌的反应。经测试的突变体分别在根平面和内球体中表现出改变的细菌和真菌群落。用突变根分泌物处理的天然土壤和细菌合成群落表现出与用野生型（WT）分泌物处理的不同的微生物特征。大多数测试的内生菌严重限制了cyp79b2b3的生长，其中一部分也损害了pyk10bglu21的生长。我们的研究结果表明，根ER体及其固有的月桂糖苷酶调节根分泌代谢产物的分布，从而影响根与微生物群的相互作用。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.