Effect of Nano-selenium on exosomes secretion associated with sperm maturation within the epididymis

IF 2.5 3区工程技术 Q1 MICROSCOPY

Micron Pub Date : 2023-09-22 DOI:10.1016/j.micron.2023.103545

Chuang Zhou , Hua Zhang , Yi Wu , Nisar Ahmed

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引用次数: 0

Abstract

Selenium is commonly used as a supplement in the poultry diet and plays an important role in male fertility. However, the effect of selenium nanoparticles (Se-NPs) on exosome secretion associated with spermatozoa in the epididymis is largely unknown. H&E staining, Immunohistochemistry, Immunofluorescence and Western blot were performed to study the effect of Se-NPs on exosomes secretion associated with sperm maturation in epididymis. The results indicated that the Se-NPs showed a significant contribution to sperm concentration by light microscopy. It was observed that there was an increase in the spermatozoa concentration in the epididymis of the treated group as compared to the control group. Furthermore, exosome secretion, the expression of tumor susceptibility gene-101 (TSG-101) and cluster of differentiation (CD-63) proteins was identified by immunochemistry, immunofluorescence assay, and western blotting. After nano-selenium treatment, the exosome markers TSG-101 and CD-63 were strong positive immunoreactivity and immunosignaling in the lumen followed by epithelial lining of the epididymis. However weak positive immunoreactivity and immunosignaling were seen of TSG-101 and CD63 in the control group. In addition, highly significant protein expression of TSG-101 and CD63 in the treated group as compared to the control group was confirmed by western blotting. In conclusion, the above findings provide rich evidence about the Se-NPs play a dynamic role in exosome secretion that might be essential for sperm motility and maturation within epididymis.

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纳米硒对附睾内精子成熟相关外泌体分泌的影响。

硒通常被用作家禽饮食中的一种补充剂，在男性生育能力方面发挥着重要作用。然而，硒纳米粒子（Se-NPs）对附睾精子外泌体分泌的影响在很大程度上是未知的。采用H&E染色、免疫组织化学、免疫荧光和蛋白质印迹等方法研究Se-NPs对附睾精子成熟相关外泌体分泌的影响。结果表明，硒纳米粒子对精子浓度有显著影响。观察到，与对照组相比，处理组附睾中的精子浓度增加。此外，通过免疫化学、免疫荧光测定和蛋白质印迹鉴定外泌体分泌、肿瘤易感基因-101（TSG-101）和分化簇（CD-63）蛋白的表达。纳米硒处理后，外泌体标志物TSG-101和CD-63在附睾管腔和上皮衬里中呈强阳性免疫反应性和免疫信号。而对照组TSG-101和CD63的免疫反应和免疫信号较弱。此外，通过蛋白质印迹证实，与对照组相比，处理组中TSG-101和CD63的蛋白质表达高度显著。总之，上述发现为Se-NPs在外泌体分泌中发挥动态作用提供了丰富的证据，这可能对精子在附睾内的运动和成熟至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Micron 工程技术-显微镜技术

CiteScore

4.30

自引率

4.20%

发文量

100

审稿时长

31 days

期刊介绍： Micron is an interdisciplinary forum for all work that involves new applications of microscopy or where advanced microscopy plays a central role. The journal will publish on the design, methods, application, practice or theory of microscopy and microanalysis, including reports on optical, electron-beam, X-ray microtomography, and scanning-probe systems. It also aims at the regular publication of review papers, short communications, as well as thematic issues on contemporary developments in microscopy and microanalysis. The journal embraces original research in which microscopy has contributed significantly to knowledge in biology, life science, nanoscience and nanotechnology, materials science and engineering.