Extracellular vesicles biogenesis and uptake concepts: A comprehensive guide to studying host-pathogen communication.

IF 2.6 2区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Microbiology Pub Date : 2024-11-01 Epub Date: 2023-09-27 DOI:10.1111/mmi.15168

Bruna Sabatke, Izadora Volpato Rossi, Abel Sana, Leticia Bassani Bonato, Marcel I Ramirez

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Abstract

The study of host-pathogen interactions has increased considerably in recent decades. This intercellular communication has been mediated by extracellular vesicles (EVs) that play an important role during the interaction. EVs are particles of lipid bilayer and described in different types of cells, eukaryotic or prokaryotic. Depending on their biogenesis they are described as exosomes (derived from multivesicular bodies) and microvesicles (derived from the plasma membrane). The EVs carry biomolecules, including nucleic acids, lipids, and proteins that can be released or internalized by other cells in different pathways (endocytosis, macropinocytosis, phagocytosis, or membrane fusion) in the process described as uptake. The balance between biogenesis and uptake of EVs could modify physiological and pathophysiological processes of the cell. This review is focusing on the dynamic roles of release and capture of EVs during host-pathogen interaction. We also do a critical analysis of methodologies for obtaining and analyzing EVs. Finally, we draw attention to critical points to be considered in EV biogenesis and uptake studies.

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细胞外小泡的生物发生和摄取概念：研究宿主-病原体通讯的综合指南。

近几十年来，对宿主与病原体相互作用的研究显著增加。这种细胞间通讯是由在相互作用中发挥重要作用的细胞外小泡介导的。EVs是脂质双层颗粒，在不同类型的细胞（真核细胞或原核细胞）中进行描述。根据它们的生物发生，它们被描述为外泌体（来源于多泡体）和微泡（来源于质膜）。EVs携带生物分子，包括核酸、脂质和蛋白质，在被描述为摄取的过程中，这些生物分子可以通过不同途径（内吞作用、大细胞吞噬作用、吞噬作用或膜融合）被其他细胞释放或内化。EVs的生物发生和摄取之间的平衡可以改变细胞的生理和病理生理过程。这篇综述的重点是EVs在宿主-病原体相互作用过程中的释放和捕获的动态作用。我们还对获得和分析电动汽车的方法进行了批判性分析。最后，我们提请注意EV生物发生和吸收研究中需要考虑的关键点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Microbiology 生物-生化与分子生物学

CiteScore

7.20

自引率

5.60%

发文量

132

审稿时长

1.7 months

期刊介绍： Molecular Microbiology, the leading primary journal in the microbial sciences, publishes molecular studies of Bacteria, Archaea, eukaryotic microorganisms, and their viruses. Research papers should lead to a deeper understanding of the molecular principles underlying basic physiological processes or mechanisms. Appropriate topics include gene expression and regulation, pathogenicity and virulence, physiology and metabolism, synthesis of macromolecules (proteins, nucleic acids, lipids, polysaccharides, etc), cell biology and subcellular organization, membrane biogenesis and function, traffic and transport, cell-cell communication and signalling pathways, evolution and gene transfer. Articles focused on host responses (cellular or immunological) to pathogens or on microbial ecology should be directed to our sister journals Cellular Microbiology and Environmental Microbiology, respectively.