Lipid peroxidation in avian semen.

Polish journal of veterinary sciences Pub Date : 2023-09-20 DOI:10.24425/pjvs.2023.145050

A Partyka, A Babapour, M Mikita, S Adeniran, W Niżański

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Abstract

The main cause of sperm chromatin damage is oxidative stress related to embryo development failure and adult infertility in mammals and also avian. Oxidative stress results in lipid peroxidation (LPO) causing cell damage. Lipid peroxidation is the oxidation of polyunsaturated fatty acids (PUFAs) in biological systems and causes changes in the physical structure and characteristics of the cell membrane. Due to the high amounts of PUFAs in the avian sperm membrane, its sperm seem susceptible to pe-roxidative damage and is a substantial factor in the fertilization capacity of sperm. The most commonly used methods for measuring LPO or its by-products, such as malondialdehyde (MDA) and 4-hydroksy-2-nonenal (4-HNE), in bird semen are based on the colorimetric method TBARS (thiobarbituric acid reactive substances) and on the use of a fluorescence probe (CC 11-BODIPY 581/591) as a marker to evaluate membrane lipid peroxidation. This review aims first to introduce LPO in avian semen and its effects on avian sperm and second to summarize the commonly applied methods of evaluating LPO and its damage in fresh and stored avian semen.

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禽类精液中的脂质过氧化。

精子染色质损伤的主要原因是与哺乳动物和鸟类胚胎发育失败和成年不育有关的氧化应激。氧化应激导致脂质过氧化（LPO）导致细胞损伤。脂质过氧化是生物系统中多不饱和脂肪酸（PUFA）的氧化，导致细胞膜的物理结构和特性发生变化。由于鸟类精子膜中含有大量的PUFA，其精子似乎容易受到氧化损伤，是影响精子受精能力的重要因素。测量鸟类精液中LPO或其副产物，如丙二醛（MDA）和4-羟基-2-壬烯醛（4-HNE）的最常用方法是基于比色法TBARS（硫代巴比妥酸反应物质）和使用荧光探针（CC 11-BODIPY 581/591）作为评估膜脂质过氧化的标记。本文首先介绍了禽类精液中的LPO及其对精子的影响，其次总结了常用的评估新鲜和储存禽类精液中LPO及其损伤的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Polish journal of veterinary sciences

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