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A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis.

Polish journal of microbiology Pub Date : 2023-09-20 eCollection Date: 2023-09-01 DOI:10.33073/pjm-2023-029
Jian Liu, Weidong Qian, Jian Wang, Yilan Bai, Yaping Gui, Luming Xia, Guohua Gong, Feifei Ge, Haixiao Shen, Xiaojing Chang, Hongjin Zhao
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Abstract

Chlamydia felis is an important zoonotic agent for humans and various animals. A recombinase-aided amplification (RAA) assay was developed for detecting C. felis. RAA can be performed in a closed tube at 39°C within 30 min. The detection limit was 10.6 copies of the C. felis plasmid DNA per reaction. No positive signals for other pathogens were detected. The coincidence rate of RAA and conventional PCR was 95.24% (20/21) and 100% (96/96) for positive and negative samples, respectively. The established RAA assay is a simple, rapid, highly sensitive, and specific method for detecting C. felis.

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一种用于检测猫衣原体的重组酶辅助扩增试验。
猫衣原体是人类和各种动物的重要人畜共患病原体。采用重组酶辅助扩增法（RAA）检测猫毛滴虫。RAA可以在39°C的封闭管中在30分钟内进行。每次反应的检测极限为10.6拷贝猫毛滴虫质粒DNA。未检测到其他病原体的阳性信号。阳性和阴性标本的RAA和常规PCR的符合率分别为95.24%（20/21）和100%（96/96）。所建立的RAA测定法是一种简单、快速、高灵敏度和特异性的检测猫科动物的方法。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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Polish journal of microbiology
Polish journal of microbiology
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