[Lercanidipine distribution in warm-blooded animals].

Q4 Medicine

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2023-01-01 DOI:10.17116/sudmed20236605147

L L Kvachakhiya, V K Shormanov

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引用次数: 0

Abstract

The aim of the study: Is to investigate the lercadipine distribution in warm-blooded animals (rats). The experimental study used rats of Wistar race. TLC, GC-MS and UV-spectrophotometry methods were used for physical and chemical analysis. Semilethal (890 mg/kg) dose of lercadipine, previously suspended in water, was injected into stomach of laboratory animals. Examined substance was isolated from the thick tissues and animals' blood by acetone, cleaned with a change of solvent and macrocolumn chromatography using 30 µm «Silasorb S-18» sorbent and acetonitrile-water (8:2) polar eluent. The analyte was identified by chromatographic behavior in the thin sorbent layer, retention time and set of positive ions in its mass spectrum, as well as by UV-spectrum. The analyte was determined quantitatively in bioactive matrix using UV-spectrophotometry. The methods were validated by criteria of linearity, selectivity, accuracy, precision, detection limits and quantitative determination. The main content of lercanidipine (mg/100 g) was determined in the stomach content (198.183±29.541), the stomach (195.312±21.579), the small intestine (47.096±3.947), the spleen (38.952±3.532) and the liver (26.211±2.232).

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[乐卡地平在温血动物中的分布]。

本研究的目的：研究乐卡地平在温血动物（大鼠）中的分布。实验研究采用Wistar种族大鼠。采用薄层色谱法、气相色谱-质谱法和紫外分光光度法进行理化分析。将预先悬浮在水中的半致死剂量（890 mg/kg）的乐卡地平注射到实验动物的胃中。用丙酮从厚组织和动物血液中分离出检查物质，用更换溶剂清洗，并使用30µm«Silasorb S-18»吸附剂和乙腈-水（8:2）极性洗脱液进行大柱色谱。分析物通过薄吸附层中的色谱行为、保留时间和质谱中的正离子组以及紫外光谱进行鉴定。用紫外分光光度法对生物活性基质中的分析物进行了定量测定。通过线性、选择性、准确度、精密度、检测限和定量测定等标准对方法进行了验证。乐卡地平（mg/100g）的主要含量测定在胃（198.183±29.541）、胃（195.312±21.579）、小肠（47.096±3.947）、脾脏（38.952±3.532）和肝脏（26.211±2.232）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sudebno-Meditsinskaya Ekspertisa Medicine-Pathology and Forensic Medicine

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The journal is concerned with the theory and practice of forensic medicine - the problems of thanatology, traumatology, toxicology, serology, forensic obstetrics, forensic dentistry, forensic psychiatry, forensic chemistry, physicotechnical methods of investigation, history of forensic medicine and some problems of criminology and legal laws related to forensic medicine. It publishes original studies by Russian authors, casuistry surveys, abstracts and reviews of Russian and foreign literature, scientific information, reports on scientific conferences.