Development and evaluation of the utility of GenoBaits Peanut 40K for a peanut MAGIC population.

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2023-09-30 eCollection Date: 2023-10-01 DOI:10.1007/s11032-023-01417-w

Ziqi Sun, Zheng Zheng, Feiyan Qi, Juan Wang, Mengmeng Wang, Ruifang Zhao, Hua Liu, Jing Xu, Li Qin, Wenzhao Dong, Bingyan Huang, Suoyi Han, Xinyou Zhang

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引用次数: 0

Abstract

Population and genotype data are essential for genetic mapping. The multi-parent advanced generation intercross (MAGIC) population is a permanent mapping population used for precisely mapping quantitative trait loci. Moreover, genotyping-by-target sequencing (GBTS) is a robust high-throughput genotyping technology characterized by its low cost, flexibility, and limited requirements for information management and support. In this study, an 8-way MAGIC population was constructed using eight elite founder lines. In addition, GenoBaits Peanut 40K was developed and utilized for the constructed MAGIC population. A subset (297 lines) of the MAGIC population at the S2 stage was genotyped using GenoBaits Peanut 40K. Furthermore, these lines and the eight parents were analyzed in terms of pod length, width, area, and perimeter. A total of 27 single nucleotide polymorphisms (SNPs) were revealed to be significantly associated with peanut pod size-related traits according to a genome-wide association study. The GenoBaits Peanut 40K provided herein and the constructed MAGIC population will be applicable for future research to identify the key genes responsible for important peanut traits.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-023-01417-w.

Abstract Image

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GenoBaits花生40K在花生MAGIC群体中的应用开发和评价。

群体和基因型数据对基因图谱至关重要。多亲本高级世代杂交（MAGIC）群体是一种用于精确定位数量性状位点的永久作图群体。此外，目标测序基因分型（GBTS）是一种强大的高通量基因分型技术，其特点是成本低、灵活性强，对信息管理和支持的要求有限。在本研究中，使用八个精英创始人系构建了一个8向MAGIC群体。此外，还开发了GenoBaits花生40K，并将其用于构建的MAGIC群体。使用GenoBaints Peanut 40K对S2阶段的MAGIC群体的一个子集（297个系）进行基因分型。此外，还对这些品系和八个亲本的荚长、宽度、面积和周长进行了分析。根据一项全基因组关联研究，共有27个单核苷酸多态性（SNPs）与花生荚大小相关性状显著相关。本文提供的GenoBaits花生40K和构建的MAGIC群体将适用于未来的研究，以鉴定花生重要性状的关键基因。补充信息：在线版本包含补充材料，网址为10.1007/s11032-023-01417-w。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.